SSeCKS对施万细胞TNF-α自分泌的作用

摘要

目的 探讨Src抑制的蛋白激酶C底物(SSeCKS)对施万细胞TNF-α自分泌的作用.方法 予TNF-α处理施万细胞及siRNA干扰SSeCKS表达后,ELISA法检测培养基上清中TNF-α的含量,蛋白免疫印迹检测SSeCKS、磷酸化p38、磷酸化JNK、总p38、总JNK的表达.结果 TNF-α可以诱导施万细胞自分泌及SSeCKS表达的增加；干扰SSeCKS表达可以抑制TNF-α的自分泌及p38与JNK通路的激活.结论 在施万细胞中,SSeCKS可能通过影响p38及JNK激活对TNF-α的自分泌发挥正向调控作用.%Objective To investigate the role of Src suppressed protein kinase C substrate ( SSeCKS) in Schwann cell' s tumor necrosis factor-α ( TNF-α) autocrine. Methods Schwann cells were treated with TNF-α and transfected with SSeCKS siRNA for 24 hours, the secreation of TNF-α was detected by enzyme linked immunosorbent assay (ELISA) , the expression of SSeCKS was analyzed by western blot. Results Treatment with TNF-α could result in induced TNF-α autocrine and SSeCKS expression. Interfering the expression of SSeCKS inhibited TNF-α autocrine and the activation of p38 and JNK pathway. Conclusions SSeCKS may have positive regulation effect on TNF-α induced autocrine in Schwann cells via the activation of p38 and JNK pathway.