[目的]观察转染pIRESneo- EGFP-BDNF的骨髓间充质干细胞(MSCs)侧脑室注射对帕金森病(PD)大鼠行为学的影响.[方法]将pEGFP(N1)-BDNF与pIRESneo进行双酶切后再连接,构建高拷贝质粒pIRESneo-EGFPBDNF,采用电穿孔法将其转染MSCs.采用6-羟多巴(6-OHDA)制备PD大鼠模型(36只),造模成功后将其随机分为3组,每组12只.B组为模型组:侧脑室注射生理盐水；C组:侧脑室注射MSCs;D组:侧脑室注射转染pIRESneoEGFP-BDNF的MSCs;A组(12只大鼠,为假手术组):以生理盐水代替6-OHDA后,侧脑室注射生理盐水.分别于侧脑室注射后2、4、8周腹腔注射阿扑吗啡(APO)诱导大鼠旋转,观察各组大鼠行为学变化.[结果]经双酶切鉴定,pIRESneo-EGFP-BDNF构建成功.侧脑室注射细胞干预PD大鼠模型后2、4、8周,大鼠旋转次数D组＜C组＜B组(P均＜0.05)；D组大鼠旋转次数明显减少,但仍较A组多.[结论]侧脑室注射转染plRESneo-EGFP-BDNF的MSCs能明显改善PD大鼠的行为能力.%Objective To observe influence on behavior of Parkinson's disease( PD) model treated by bone mesen-chymal stem cells ( MSCs) modified by plasmid pIRESneo-EGFP-BDNF. Methods pEGFP-BDNF and pIRESneo were double enzymed and then were reconstructed into pIRESneo-EGFP-BDNF which was transfected to MSCs with electroproa-lion. 36 rat models of PD were set up by 6-OHDA and divided into three groups randomly; group B( group model): inlrac-erebrovenlricular injection of saline; group C: intracerebroventricular injection of bone MSCs; group D: intracerebroventric-ular injection of bone MSCs modefied by pIRESneo-ECFP-BDNF. Group A (12 rats, sham opreation groups): saline instead of 6-OHDA, than intracerebroventricular injection of saline. The rotating behavior of rat models induced by Apomor-phine intraperitoneally which transplanting bone MSCs or MSCs modified by plasmid pIRESneo-EGFP-BDNF through cerebral lateral ventricle after 2, 4 and 8 weeks. Results Pbsmids pIRESneo-EGFP-BDNF were constructed successfully, which was identified by double digestion. The rotation numbers ( r/min) of D group < C group < B group in the 2th, 4th and 8th week after transplanting were significant decrease compared with PD group ( all P < 0.05). Those of D group were exespecially significant decreased, but were increased compared with A group. Conclusion The behavior of rat with PD was improved significantly while transplanting bone MSCs modified by BDNF through cerebral lateral ventricle.
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