肺癌细胞及组织中miR-181a、miR-200a、miR-200c表达变化及意义

摘要

Objective To observe the expression and significance of miR-181a, miR-200a, miR-200c in lung cancer tissues and cells.Methods RT-PCR method was used to detect the expression of miR-181a, miR-200a, miR-200c in lung cancer cell lines NCI-H1299, NCI-H358, H460, A549 and normal human bronchial epithelial cell line HBE , and in the lung cancer tissues and adjacent tissues .To analyze the relationship between the expression of miR-181a, miR-200a, miR-200c and clinicopathological parameters of lung cancer by means of Mann-Whitney.Results miR-181a expression in NCI-H1299, NCI-H358 and A549 were lower than that in HBE (all P<0.05).miR-200a expression in NCI-H1299, NCI-H358, H460 and A549 were lower than that in HBE (all P<0.05).miR-200c expression in NCI-H358 and H460 were higher than that in HBE (all P<0.05).The expression of miR-181a and miR-200a were no significant differences between lung cancer tissues and cancer-adjacent tissues (all P>0.05).miR-200c expression in lung cancer tissues was higher than that in cancer-adjacent tissues (P<0.01).miR-200a expression in the lung cancer patients with lymphatic metastasis was lower than that in lung cancer patients without lymphatic metastasis (P<0.01).Conclusions The expression of miR-181a, miR-200a were generally decreased , while miR-200c was generally increased in lung cancer cell lines and tissues , which maight be related to the occur of lung cancer .%目的：观察肺癌细胞及组织中 miR-181a、miR-200a、miR-200c 的表达变化，并探讨其临床意义。方法选择人肺癌细胞系NCI-H1299、NCI-H358、H460、A549及人正常支气管上皮细胞HBE，10例肺癌患者的癌组织及其相应癌旁组织。采用RT-PCR法检测各细胞系及组织中的miR-181a、miR-200a、miR-200c，并分析miRNA表达与临床参数的关系。结果 NCI-H1299、NCI-H358、A549细胞中miR-181a相对表达量均低于HBE细胞，NCI-H1299、NCI-H358、H460、A549细胞中miR-200a相对表达量均低于HBE细胞，NCI-H358、H460细胞中miR-200c相对表达量均高于HBE细胞，P均＜0．05。肺癌及癌旁组织中miR-181a和miR-200a相对表达量比较，P均＞0．05；肺癌组织中miR-200c相对表达量高于癌旁组织，P＜0．01。有淋巴结转移的肺癌患者miR-200a相对表达量低于无淋巴结转移者，P＜0．01；其余miRNA相对表达量与临床病理参数均无关，P均＞0．05。结论 miR-181a、miR-200a在肺癌细胞株中表达普遍降低，而miR-200c在肺癌细胞及组织中表达普遍升高，可能与肺癌的发病有关。