肺癌细胞与组织中miR-100、miR-148a的表达变化及临床意义

摘要

Objective To investigate the expression and clinical significance of miR-100 and miR-148a in lung cancer cells and tissues.Methods RT-PCR method was used to detect the expression of miR-100 and miR-148a in lung cancer cell lines NCI-H1299, NCI-H358, H460, A549 and normal human bronchial epithelial cell line HBE, and in the lung cancer tissues and adjacent tissues.To analyze the relationship between the expression of miR-100 and miR-148a and clini-copathological parameters of lung cancer.Results Compared with HBE cells, miR-100 expression in NCI-H1299, NCI-H358 and A549 increased significantly, decreased in H460, all P<0.05; the expression of miR-148a in NCI-H358 and H460 increased, decreased in A549, all P<0.05.Compared to non-tumor tissues, miR-100 expression was reduced in lung cancer, but the difference was not statistically significant (P=0.400);while the expression of miR-148a was signifi-cantly increased (P=0.036).miR-100 expression in lung cancer tissue with lymph node metastasis was lower than that in lung cancer tissue without lymph node metastasis (P=0.016).Conclusions The expression of miR-148a, miR-100 were aberrantly changed in lung cancer cells.miR-100 expression decreased in lung cancer tissues, miR-148a increased, which both maight be involved in the tumorigenesis of lung cancer.%目的：观察微小RNA 100（miR-100）、微小RNA 148a（miR-148a）在肺癌细胞与组织中的表达，并探讨其临床意义。方法采用RT-PCR方法检测人肺癌细胞系NCI-H1299、NCI-H358、H460、A549及人正常支气管上皮细胞系HBE，以及肺癌及其癌旁组织中miR-100、miR-148a表达；分析肺癌组织中miR-100、miR-148a表达与其临床病理参数的关系。结果与HBE细胞相比，miR-100在NCI-H1299、NCI-H358及A549细胞中表达升高，在H460细胞表达降低，P均＜0．05；miR-148a在NCI-H358、H460细胞中表达升高，在A549细胞中表达降低，P均＜0．05。与癌旁组织相比，肺癌组织中miR-100表达降低，但差异无统计学意义（P＝0．400）；miR-148a表达升高（P＝0．036）。肺癌组织中miR-100、miR-148a表达水平与患者性别、年龄、病理类型无关；有淋巴结转移者肺癌组织中miR-100表达水平较无淋巴结转移者低（P＝0．016）。结论 miR-100、miR-148a在肺癌细胞中表达异常；在肺癌组织中，miR-100表达降低，miR-148a表达增加，二者可能参与肺癌的发生、发展。