七氟醚预处理对大鼠肝缺血再灌注致肺损伤的干预作用及机制探讨

摘要

Objective To observe the intervention of sevoflurane pretreatment on rat pulmonary injury caused by liver ischemia reperfusion and to explore its mechanism .Methods A total of 30 healthy Wistar rats were randomly divided into sham-operation group , the model group and the sevoflurane group , with 10 rats in each group .Models of liver ischemia reperfusion were established in the model group and sevoflurane group , and rats of the sevoflurane group were given inhala-tion of sevoflurane for 30 min before modeling; while in the sham-operation group , only porta hepatis was separated but blood supply was not blocked .Rats were scarified at 60 min after reperfusion , and the serum alanine aminotransferase ( ALT) , aspertate aminotransferase ( AST) and tumor necrosis factor-α( TNF-α) were detected; part of right upper lobe was used to measure the wet/dry weight ratio ( W/D ratio) and part was used to make paraffin section for pathological ob-servation;and left lung tissue was taken to determine malondialdehyde ( MDA) and MMP-9 mRNA.Results Lung tissue of the sham-operation group was normal in structure under microscopy , while damage degree of lung tissue in the sevoflu-rane group was reduced as compared with that of the model group .Serum ALT, AST and TNF-αof the model group and sevoflurane group were increased as compared with those of the shan-operation group (all P<0.05); while serum ALT, AST and TNF-αof the sevoflurane group was lower than that of the model group (all P<0.05).W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the model group and sevoflurane group were increased as compared with those of the sham-operation group ( all P<0.05 ); W/D ratio, MDA content and relative expression of MMP-9 mRNA in the lung tissues of the sevoflurane group were lower than those of the model group (all P<0.05).Con-clusion Sevoflurane can alleviate the pulmonary injury caused by liver ischemia reperfusion , whose mechanism may be re-lated to the lower levels of TNF-αand inhibition of MMP-9 mRNA expression, etc.%目的：观察七氟醚预处理对肝缺血再灌注致肺损伤的干预作用，并探讨其机制。方法健康Wistar大鼠30只，随机分为假手术组、模型组、七氟醚组，每组10只。模型组与七氟醚组制作部分肝缺血再灌注模型，其中七氟醚造模前给予七氟醚吸入30 min；假手术组仅游离肝门，但不阻断血供。于再灌注60 min时处死大鼠，测血清ALT、AST、TNF-α；取部分右肺上叶组织用于测算湿重与干重比值（W／D），部分制作石蜡切片用于病理观察；取左肺组织，测定丙二醛（ MDA）及MMP-9 mRNA。结果光镜下假手术组肺组织结构未见异常；七氟醚组肺组织损伤程度较模型组减轻。模型组、七氟醚组血清ALT、AST、TNF-α水平升高，与假手术组相比，P均＜0．05；七氟醚组血清ALT、AST、TNF-α水平低于模型组（P均＜0．05）。模型组、七氟醚组肺组织W／D、MDA含量及MMP-9 mRNA相对表达量均升高，与假手术组相比，P均＜0．05；七氟醚组肺组织W／D、MDA含量及MMP-9 mRNA相对表达量低于模型组（P均＜0．05）。结论七氟醚可减轻肝缺血再灌注导致的肺损伤，机制可能与降低TNF-α水平、抑制MMP-9 mRNA表达等有关。