垂体腺瘤组织中 NF-κB、MMP-9、MICA 的表达变化及意义

摘要

Objective The aim of this study was to examine the expression levels of NF-κB, MMP-9, and MICA and to explore its significance .Methods We examined the association between NF-κB, MMP-9, and MICA by immunohisto-chemical staining, Quantitative real-time PCR.Results The positive rate of NF-κB were 100%(40/40), 30%(6/20)in pituitary adenomas and normal brain tissue .The positive rate of MMP-9 were 100%(40/40),15%(3/20)in pituitary ade-nomas and normal brain tissue .The positive rate of MICA were 100%(40/40), 0 in pituitary adenomas and normal brain tissues(P<0.05).Relative to express the NF-κB mRNA were 13.045 ±6.2, 2.04 ±2.067 in pituitary adenomas and normal brain tissues.Relative to express the MMP-9 mRNA were 28.77 ±7.661, 2.369 ±1.46 in pituitary adenomas and normal brain tissues.Relative to express the MICA mRNA were 231.817 ±41.046, 5.062 ±3.849 in pituitary adenomas and normal brain tissues(P<0.05).The expression levels of NF-κB, MMP-9, and MICA were positively related in pa-tients with pituitary adenomas (r=0.368,0.345,P<0.05).Conclusions NF-κB, MMP-9, and MICA are highly ex-pressed in pituitary adenomas tissues .High levels of NF-κB by promoting the expression of MMP-9 and MICA promote the development of pituitary adenomas .%目的：观察垂体腺瘤组织中NF-κB、基质金属蛋白酶9（MMP-9）、MHC-Ⅰ类链分子相关抗原A（MICA）的表达变化，并探讨其意义。方法采用免疫组化法对垂体腺瘤组织和正常脑组织中的NF-κB蛋白、MMP-9蛋白及MICA蛋白进行检测；采用qRT-PCR法对垂体腺瘤组织及正常脑组织中的NF-κB mRNA、MMP-9 mRNA、MICA mRNA进行检测。结果垂体腺瘤与正常脑组织中NF-κB蛋白阳性表达率分别为100％（40／40）、30％（6／20） ， MMP-9蛋白阳性表达率分别为100％（40／40）、15％（3／20），MICA蛋白阳性表达率分别为100％（40／40）、0；两组比较，P均＜0．01。垂体腺瘤与正常脑组织中NF-κB mRNA相对表达量分别为13．045±6．200、2．040±2．067， MMP-9 mRNA相对表达量分别为28．770±7．661、2．369±1．460，MICA mRNA 相对表达量分别为231．817±41．046、5．062±3．849；两组比较，P均＜0．05。 NF-κB蛋白与MICA蛋白、MMP-9蛋白表达均呈正相关（ r分别为0．368、0．345，P均＜0．05）。结论 NF-κB、MMP-9及MICA在垂体腺瘤组织中高表达，NF-κB可能通过促进MMP-9及MICA表达而促进垂体腺瘤发生、发展。