雷米普利对慢性心衰大鼠肾间质纤维化及PDGF-B、CTGF表达的影响

摘要

Objective To observe the effects of ramipril on the renal interstitial fibrosis,expression of platelet derived growth factor (PDGF-B)and connective tissue growth factor (CTGF)in the renal tissues of rats with chronic heart failure. Methods The 30 male Wistar rats were randomly divided into the Sham group (group A,10 rats),model group (group B,10 rats)and the ramipril group (group C,10 rats).The chronic heart failure model was established by the renal upper abdominal aorta coarctation.After the model was successfully established,the rats in the group C were administrated with 1 mg/(kg·d)ramipril for 4 weeks,while rats in groups A and B were administrated with normal saline for 4 weeks.The degree of renal fibrosis was detected under light microscope by HE and Masson staining.The protein and mRNA expression levels of PDGF-B and CTGF were respectively detected by immunohistochemistry and RT-PCR.Results Compared with group A,the renal fibrosis was significantly found in the groups B and C,and group B was more severe than the group C. The protein and mRNA expression levels of PDGF-B and CTGF were significantly higher in the groups B and C than that those of group A,and moreover,group B was higher than group C (all P <0.01).Conclusion Ramipril can effectively decrease the renal interstitial fibrosis,expression levels of PDGF-B and CTGF in rats with chronic heart failure.%目的：观察雷米普利对慢性心力衰竭大鼠肾间质纤维化及肾组织血小板源生长因子B（PDGF-B）、结缔组织生长因子（CTGF）表达的影响。方法将30只雄性 Wistar 大鼠随机分为假手术组（A 组）、模型组（B 组）和雷米普利组（C 组）各10只。B、C 组大鼠通过肾上腹主动脉部分缩窄法建立大鼠慢性心力衰竭模型，A 组大鼠只分离肾上腹主动脉而不结扎。术后 C 组大鼠给予雷米普利1 mg／（kg·d）灌胃，A、B 两组大鼠给予等量生理盐水灌胃，常规喂养4周。取各组大鼠肾组织行 HE、Masson 染色观察病理变化，用免疫组化法检测各组肾脏组织中PDGF-B、CTGF 蛋白表达，用 RT-PCR 法检测各组大鼠肾脏组织中 PDGF-B、CTGF mRNA 表达。结果与 A 组相比，B、C 组大鼠肾脏出现明显纤维化改变，其中 C 组纤维化病变程度较 B 组轻。B、C 组大鼠 PDGF-B、CTFG 蛋白和 mRNA 表达均高于 A 组，且 B 组高于 C 组（P 均＜0．01）。结论雷米普利可降低心衰大鼠肾间质纤维化程度及肾组织 PDGF-B、CTFG 表达。