SIRT1在结直肠癌组织中的表达及其与 MDR-1的关系

摘要

目的：探讨沉默信息调节因子1（SIRT1）在结直肠癌组织中的表达及其与多药耐药相关蛋白1（MDR-1）的关系。方法选择结直肠癌患者162例，取手术切除的癌组织，采用免疫组化Envision法观察SIRT1、MDR-1的阳性表达，分析结直肠癌组织SIRT1阳性表达与患者临床病理参数及MDR-1阳性表达的关系。结果结直肠癌组织中SIRT1、MDR-1阳性表达率分别为66．0％（107／162）和59．3％（96／162）。结直肠癌组织SIRT1阳性表达与患者性别、肿瘤组织分化程度、临床分期及淋巴结转移无关（P均＞0．05），与肿瘤长径有关（P＜0．05）。结直肠癌组织SIRT1阳性表达与MDR-1阳性表达呈正相关（r＝0．506，P＜0．01）。结论结直肠癌组织中SIRT1阳性表达较高，其阳性表达与MDR-1阳性表达呈正相关关系。%Objective To investigate the expression of silent information regulator 1 ( SIRT1) in colorectal carcinoma (CRC) and its relationship with and multidrug resistance-related protein 1 (MDR-1).Methods We used the imunohisto-chemistry method to detect the expression of SIRT 1 and MDR-1 and analyzed their relationships with clinical data in 162 specimens of CRC.Results SIRT1 and MDR-1 expression was detected in 107 (66.0%) and 96 (59.2%) out of 162 colorectal adenocarcinomas , respectively.Statistically, the expression of the SIRT1 protein was not associated with clinical and pathological parameters , including sex, tumor stage, lymph node metastasis and tumor differentiation (all P>0.05), but was associated with tumor size (P<0.05).In addition, the positive expression of SIRT1 and MDR-1 in CRC was posi-tively correlated with each other (r=0.506, P<0.01).Conclusion SIRT1 is highly expressed in the CRC and its ex-pression is positively correlated with MDR-1 expression.