Objective To observe the gene polymorphisms of vascular endothelial growth factor ( VEGF ) rs833061 and rs3025039 and to investigate their interaction with smoking in patients with non-alcoholic fatty liver disease ( NAFLD) . Methods Genotypes of VEGF rs833061 and rs3025039 in 341 patients with NAFLD (NAFLD group) and 246 control subjects ( control group ) were examined by polymerase chain reaction-based restriction fragment length polymorphism ( PCR-RFLP) .The unconditional logistic regression ( ULR) was performed to analyze the relationships between the geno-types and susceptibility to NAFLD .The interactions between genotypes of VEGF rs 833061 and rs3025039 and smoking in inducing NAFLD were explored by generalized multifactor dimensionality reduction ( GMDR) and unconditional Logistic re-gression method.Results The risk of NAFLD in VEGF rs833061 with genotype C (CC+CT) was higher than that of car-riers without C (TT) (all P<0.01), and the risk of NAFLD in rs3025039T gene carriers (TT+CT) was higher than that of carriers without genotype T (CC) (all P<0.01).Gene polymorphism of VEGF rs833061 had interactions with smoking in the process of NAFLD (P<0.01).The risk of NAFLD in rs833061 CC/CT genotype of smoking individuals was 4.93 times higher than that of TT genotype of non-smoking individuals .Conclusions VEGF gene polymorphism is associated with the risk of NAFLD.VEGF rs833061 and smoking have synergistic effects in the process of inducing NAFLD.%目的 观察非酒精性脂肪肝( NAFLD)患者血管内皮生长因子( VEGF)基因rs833061、rs3025039位点多态性,并探讨其多态性及吸烟在诱发NAFLD中的交互作用. 方法 选择NAFLD患者341例( NAFLD组)、健康体检者246例(对照组) ,采用PCR-RFLP法分析VEGF基因rs833061、rs3025039位点基因型,非条件Logistic回归分析法分析各基因型与NAFLD易感性的关系,非条件Logistic回归分析法和广义多因子降维法分析VEGF基因多态性及吸烟在诱发NAFLD中的交互作用. 结果 VEGF基因rs833061位点C基因携带者( CC+CT)罹患NAFLD的风险较非C基因携带者(TT)升高(P均<0.01),rs3025039位点T基因携带者(TT+CT)患病风险较非T基因携带者(CC)风险升高(P均<0.01). VEGF基因rs833061位点多态性与吸烟可能在NAFLD发病过程中存在相加作用(P<0.01),rs833061位点CC/CT基因型吸烟个体罹患NAFLD风险是携带TT基因型非吸烟个体的4.93倍. 结论 VEGF基因多态性与NAFLD发病有关,VEGF基因rs833061位点多态性与吸烟在诱发NAFLD过程中具有协同效应.
展开▼
