VEGF-C在口腔颌面部淋巴管瘤组织中表达变化及其对人脐静脉内皮细胞增殖的影响

摘要

目的 探讨血管内皮生长因子C(VEGF-C)在口腔颌面部淋巴管瘤(OML)发生、发展中的作用.方法 收集OML组织(OML组)和瘤旁正常组织(Control组)各54例份,采用qRT-PCR法检测组织中VEGF-C mRNA相对表达量,采用免疫组化法检测VEGF-C蛋白阳性表达率.将体外培养的人脐静脉内皮细胞ECV304随机分为两组,分别转染sh-VEGF-C质粒(观察组)和对照质粒sh-control(对照组),转染48 h时,分别采用qRT-PCR法和Western blotting法检测VEGF-C mRNA和蛋白相对表达量,采用MTT法检测细胞增殖能力(以吸光度值表示).结果 OML组与Control组VEGF-C mRNA相对表达量分别为1.061 ±0.284、0.801±0.098,VEGF-C蛋白阳性表达率分别为84.10％ (42/54)、10.13％ (5/54),两组比较P＜0.05或＜0.01.观察组与对照组VEGF-C mRNA相对表达量分别为0.200±0.058、0.967±0.017,VEGF-C蛋白相对表达量分别为0.319±0.021、0.965±0.039,吸光度值分别为0.393±0.015、0.975±0.004,两组比较P均＜0.01.结论 VEGF-C在OML组织中高表达;降低VEGF-C表达可抑制人静脉内皮细胞增殖.%Objective To explore the expression of vascular endothelial growth factor-C (VEGF-C) in the occurrence and development of oral and maxillofacial lymphangioma (OML).Methods The tumor tissues (OML group) and the adjacent normal tissue (control group) from OML patients were collected.The real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of VEGF-C mRNA in the tissues.Immunohistochemistry (IHC) was used to detect the positive rate of VEGF-C protein expression in the tissues.ECV304 cells cultured in vitro were randomly divided into two groups:the observation group which was transfected with sh-VEGF-C plasmid and the control group transfected with control plasmid sh-control.After 48-hour transfection,qRT-PCR and Western blotting were used to detect the relative expression of VEGF-C protein,and MTT assay was used to detect the proliferation of the cells (absorbance value).Results In the OML group and control group,the expression of VEGF-C mRNA was 1.061 ± 0.284 and 0.801 ± 0.098,respectively;the positive expression rates of VEGF-C protein was 84.10％ (42/54) and 10.13％ (5/54),respectively (P ＜ 0.05 or P ＜ 0.01).In the observation group and control group,the relative expression levels of VEGF-C mRNA were 0.200 ±0.058 and 0.967 ±0.017,respectively;the relative expression levels of VEGF-C protein were 0.319 ±0.021 and 0.965 ± 0.039,respectively;the A values were 0.393 ± 0.015 and 0.97 ± 0.004,respectively;significant differences were found between these two groups (all P ＜ 0.01).Conclusions VEGF-C is highly expressed in the OML tissues,and decreasing VEGF-C can inhibit the proliferation of human umbilical vein endothelial cells.