绞股蓝总皂苷对胶质母细胞瘤细胞株U87细胞形态、迁移能力的影响

摘要

目的 观察不同剂量绞股蓝总皂苷(Gyp)对胶质母细胞瘤(GBM)细胞株U87细胞形态、迁移能力的影响,并探讨其机制.方法 取对数生长期U87细胞,分别加入10、5 mg/mL的Gyp溶液100 μL及等量PBS(分别计为A、B、C组),常规培养细胞.比较各组细胞形态、迁移能力及IL-6蛋白.结果 A组细胞生长慢、体积小、固缩现象非常明显,细胞贴壁现象接近消失;B组细胞生长缓慢,体积缩小,胞质固缩,部分细胞甚至破裂死亡,细胞贴壁现象减弱;C组细胞生长规则,轮廓清晰,形态饱满,胞体透亮,贴壁生长良好.A、B、C组细胞迁移覆盖区域占原有划痕区的54.8％、82.4％、98.6％,两两比较,P均＜0.05.A、B、C组穿膜细胞个数分别占19.8％、58.9％、100％,两两比较,P均＜0.05.A、B、C组IL-6蛋白水平分别为小量、中等量、多量,两两比较,P均＜0.05.结论 Gyp可抑制U87细胞生长及减弱细胞迁移能力,尤以10 mg/mL的Gyp效果更佳,机制可能与其可抑制IL-6蛋白表达有关.%Objective To observe the effects of different doses of gypenosides (Gyp) on the migration and cell morphology of human glioblastoma U87 cells in vitro and to explore the mechanism.Methods U87 cells in the logarithnic growth phase were added with 100 μL of 10 and 5 mg/mL Gyp solution and the same volume of PBS (referred as groups A,B,and C),and then given the routine cultivation.The cell morphology,migration ability,and interleukin-6 protein levels were compared in each group.Results In the group A,the cells grew very slowly with small volume,the pyknosis was very obvious and the phenomenon of cell attachment disappeared nearly.In the group B,the cells grew slowly with reduced volume and cytoplasm pyknosis,some cells even ruptured and died,and the cell attachment decreased.In the group C,the cell growth was regular with clear outline and full-shape,the cell body was translucent,and the adherent growth was good.The area of cell migration in the groups A,B,and C accounted for 54.8％,82.4％,and 98.6％ of the original scratch area (all P＜0.05);the number of transmembrane cells in the groups A,B,and C accounted for 19.8％,58.9％,and 100％ (all P ＜ 0.05);the protein levels of IL-6 in the groups A,B,and C were high,moderate,and low,respectively;significant differences were found between every two groups (all P ＜ 0.05).Conclusion The Gyp inhibits the growth and metastasis ability of U87 cells with the best effect at 10 mg/mL Gyp,and the mechanism may be associated with the inhibition of IL-6 protein in U87 cells.