高效液相色谱法测定苹果醋饮料中糖精钠含量

摘要

A method of determination of saccharin sodium in apple vinegar beverage by high performance liquid chromatography (HPLC) was proposed. After pretreatment, the samples were separated in a C18 column and the content of saccharin sodium in 6 different apple vinegar beverages was determined according to the retention time and the standard curve. The results showed that the calibration curve within the range of 10 ～ 200 μg/mL showed a good linearity and desirable correlation coefficient(R2 =0. 9992). The limit of detection of the method was 0. 008g/kg and the individual average recovery at 3 different spiked concentration levels was about 96％ with the relative standard deviations (RSD) no more than 5％. The established method is sensitive and accurate and can be used to determine the content of saccharin sodium in vinegar drinks.%建立了高效液相色谱(HPLC) 法测定苹果醋饮料中糖精钠的检测方法.样品通过前处理后,经C18反相色谱柱分离,根据保留时间定性、标准曲线定量测定了6种市售苹果醋饮料中糖精钠的含量.结果 表明: 糖精钠在10 ～ 200μg/mL浓度范围内,线性关系(R2 =0. 9992) 好,最低检出限为0. 008 g/kg,3个不同加标浓度的平均回收率均约为96％,RSD不超过5％.该方法精密度、重复性和准确度均良好,可用于醋饮料中糖精钠含量的测定.