转基因番茄定性PCR检测

摘要

Based on the specific structures of tomato endogenous reference gene PG and general genes of transgenic tomato, such as CaMV 35S promoter, NOS terminator, npt - Ⅱ gene and antisense EFE gene, five pairs of primers were designed for qualitative PCR. The results showed that the target DNA sequences specific to every transgenic tomato could be detected, which indicated that the primers had high specificity and accuracy, and could identify transgenic tomatoes specifically.%根据番茄内参基因PG和转基因番茄中常见的CaMV 35S启动子、NOS终止子、npt-Ⅱ基因、反义EFE基因等的特异性结构,设计5对定性PCR引物,经定性PCR检测,可以准确地检测出目的基因,说明这5对引物具有较高的特异性和准确性,可以用于转基因番茄及其产品的定性PCR检测.