首页> 中文期刊> 《山东农业科学》 >紫叶李叶片花色素及花色苷HPLC定量分析

紫叶李叶片花色素及花色苷HPLC定量分析

         

摘要

利用高效液相色谱(HPLC)法首次定量分析了紫叶李鲜叶片中芍药色素(Peonidin)、花青素3,5-二葡萄糖苷(Cyanidin 3,5-di-O-glucoside)、天竺葵色素3,5-二葡萄糖苷(Pelargonidin 3,5-di-O-glucoside)、芍药色素3-葡萄糖苷(Peonidin 3-O-glucoside)、飞燕草素(Delphinidin)、花青素(Cyanidin)和锦葵色素(Malvidin)的存在.花青素3-葡萄糖苷(Cyanidin 3-O-glucoside)、芍药色素和色素12是紫叶李鲜叶片中三种重要色素,其相对含量均超过检测到色素总含量的10%,分别占检测到色素总含量的27.54%、24.08%和14.95%,鲜叶片中实际含量分别为3.396、2.969、2.108 mg/g.除芍药色素3-葡萄糖苷极微量外,花青素3,5-二葡萄糖苷、锦葵色素、飞燕草素、天竺葵色素3,5-二葡萄糖苷和花青素在鲜叶片中的含量分别为0.005、0.0075、0.0185、0.3590、0.0360 mg/g.其它28种色素的结构和含量需要进一步分析.%The contents of Peonidin, Cyanidin 3,5 - di - 0 - glucoside, Pelargonidin 3 ,5 - di - 0 -glucoside, Peonidin 3 - 0 - glucoside, Delphinidin, Cyanidin and Malvidin in fresh leaves of Prunus cerasif-era var. Atropurpurea were quantitatively analyzed by HPLC. Cyanidin 3 - 0 - glucoside, Peonidin and No. 12 pigment were the principal pigments with the relative contents of 27.54% , 24.08% and 14.95% respectively, whose actual contents in fresh leaves were 3. 396, 2.969 and 2.108 mg/g respectively. The contents of Cyanidin 3,5 - di - 0 - glucoside, Malvidin, Delphinidin, Pelargonidin 3,5 - di - 0 - glucoside and Cyandin in fresh leaves were 0.005, 0.0075, 0.0185, 0.359 and 0.036 mg/g respectively, except Peonidin 3 - 0 -glucoside, whose content was very low. The structure and content of the other 28 kinds of pigments needed further analysis.

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