CPPU 处理对小麦分蘖影响的研究

摘要

以冬小麦潍麦8号为试材，研究不同浓度CPPU处理的小麦萌发胚以及种子所获植株的分蘖及植株发育变化。结果显示，CPPU可以促进小麦萌发胚形成的幼苗产生分蘖，当CPPU浓度小于6μmol・ L-1时，产生的分蘖数随着CPPU浓度的升高而增多，而且随着处理时间的延长，二级分蘖苗的比率也增大；但是CPPU浓度高于10μmol・ L-1时会对幼苗的生长造成毒害。在一定浓度范围内，CPPU浸种处理的有效穗数也是随着浓度的升高而增多。 CPPU处理后的小麦幼苗生长素合成基因TaYUCCA10的表达比对照增多，且随处理时间的延长表达量呈上升趋势。%The winter wheat cultivar Weimai 8 was used to study the effects of different concentrations of CPPU on wheat tillering and plant development in the laboratory and field .The results showed that CPPU could induce tillering of wheat seedling .When the CPPU concentration was below 6 μmol・ L-1 , the number of tillers increased with the increase of CPPU concentration .And with the extension of treating time , the ratio of seedlings with secondary tillering increased .But there was pernicious effects on seedlings when the CPPU concentration was above 10 μmol・ L-1 .The number of productive ear increased with the increase of CPPU concentration in certain concentration range .The expression quantity of auxin synthesis gene , TaYUCCA10, was higher after treated with CPPU than that of control , and raised with the extension of treating time .