鲁西黑头羊mtDNAD－loop区遗传多样性与遗传分化研究

摘要

Luxi black head sheep is an excellent cultivated breed with high yield of meat and high prolif-icacy.To assess the genetic diversity of Luxi black head sheep on mtDNA and obtain the molecular evidence on breed identification,the hypervariable regions of mtDNA in Luxi black head sheep,Dorper and small tail Han sheep were sequenced,and their genetic diversity,genetic differentiation and genetic distance were ana-lyzed.The results indicated that the genetic diversity among three breeds was rich and the degree of genetic differentiation was high (Fst as 0.3240 ~0.5909).The genetic distance between Luxi black head sheep with Dorper was 0.1187,which reached the value between species;and that with small tail Han sheep was 0.0654,which reached the value between subspecies.The results provided data support for the breed identifi-cation of Luxi black head sheep.%鲁西黑头羊是一个优良的绵羊培育品种，具备高产肉力和高繁殖力特征。为了评估这个品种线粒体 DNA 的遗传多样性，同时获得该新品种界定的分子证据，本研究对鲁西黑头羊、杜泊羊和小尾寒羊 mtD-NA 的高变异区进行了测序，分析了它们的遗传多样性和遗传分化，并计算了鲁西黑头羊与其它两个品种间的遗传距离。结果表明：三个品种的遗传多样性较为丰富，遗传分化程度较高（Fst 为0．3240～0．5909）；鲁西黑头羊与杜泊羊之间的遗传距离为0．1187，达到了种间的遗传距离，与小尾寒羊之间的遗传距离为0．0654，达到了亚种间的水平。以上结果为鲁西黑头羊的品种界定提供了数据支持。