利用特异性 SNP 位点鉴定花生杂交 F1代真假杂种

摘要

The peanut varieties,Luhua 14,Tifrunner and Silihong,were used as parents to make hybrid combinations through reciprocal cross.A set of technology for identifying the true F1 hybrid by restriction en-zyme digestion was established using the special SNP loci of parents.The results showed that the digestion band patterns of true hybrids were consistent with those of parents,while false hybrids only showed the female parent band patterns.The true hybrid rate of the four combiniations ranged from 10.5% to 36.7%.There-fore,this technology could be used to identify the true F1 hybrid of peanut effectively.%以鲁花14、Tifrunner 和四粒红为亲本通过正、反交获得的杂种 F1代群体为材料，结合亲本间特异性单核苷酸多态性（Single Nucleotide Polymorphism，SNP）位点，建立起一套利用限制性内切酶酶切方法鉴定花生杂交 F1代真假杂种的技术。结果表明，真杂种表现出父、母本带型，而假杂种只表现出母本带型；4个组合中 F1代真杂种率为10．5％～36．7％。因此，该技术能够有效应用于花生杂交 F1代的真假杂种鉴定。