杂种落叶松不同子代针叶内抗氧化酶活性的比较

摘要

为研究不同品种杂种落叶松针叶内抗氧化酶活性的差异,用紫外分光光度法对日本落叶松、兴安落叶松和长白落叶松的杂交后代,共2代7个家系的杂种落叶松针叶内的过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性及其差异进行比较分析.结果表明:2代7个家系杂种落叶松针叶内的CAT、POD和SOD的活性差异极显著(P＜0.01),F1代CAT、SOD的活性普遍高于F2代,POD活性低于F2代.在抗氧化酶方面,F1代杂种优势高于F2代,F1代日3×兴2、F2代日12×兴9等具有较好的潜在抗虫性;结合其他抗虫生理指标,该研究结果在选育落叶松抗虫品种过程中有参考价值.%Antioxidases are important defensive substance in plants, and can maintain balance of oxidative metabolisms in vivo to ensure healthy growth of plants. In this study, the activities of superoxide dismutase (SOD) , catalase (CAT) and peroxidase (POD) in needles of hybrids between Larix kaempferi, L. Gmelinii and L. Olgensis were assayed with UV-spectrometry to investigate variation in the enzymes among the hybrids. The results showed that there were significant difference (P < 0. 01) in SOD, CAT and POD activities among 7 families hybrids of two generations. Though POD activity of Fl generation was lower than F2 generation, activities of the CAT or SOD were higher than F2 generation, suggesting that heterosis of Fl generation was greater than F2 generation. Additionally, some hybrids, such as Fl generation of L. Kaempferi 3×L. Gmelinii 2, and F2 generation of L. Kaempferi 12×L. Gmelinii 9, expressed strong resistance to pests and could be used as resistance resources against larch pests.