In this work, a full-length Cdna sequence of APETALA2 gene was obtained from petals of Paeonia suffruticosa ' Zhaofen ' using RT-PCR and RACE, named PsAP2 ( GenBank accession No. HM167511). Sequence analysis indicated that PsAP2 is 2 138 bp in full length, containing a 5'-untranslated region (5'-UTR) of 47 bp, a 3'-UTR of 557 bp, and an opening reading frame (ORF) of 1 533 bp encoding a 510 predicted ammo acids,and belongs to AP2 family. Sequence alignment and phylogenetic analysis revealed that PsAP2 shared more than 70% homology with Vitis vinefera. Relative Real-Time PCR analysis indicated that PsAP2 expressed in roots, stems, leaves and four floral organs. The expression in petals was the highest and the expression in leaves was the lowest.%以牡丹品种'赵粉'为试材,采用RT-PCR和RACE方法从花瓣中获得1个牡丹APETALA2基因cDNA全长,命名为PsAP2,GenBank登录号为HM167511.序列分析结果表明:PsAP2全长2138bp,包含47bp的5'非编码区、557bp的3'非编码区和1个长度为1533bp编码510个氨基酸的开放阅读框.氨基酸序列分析显示该基因属于AP2家族.序列比对和系统进化分析表明,PsAP2与葡萄的亲缘关系最近,相似性达70%以上.相对荧光定量PCR分析表明,PsAP2在根、茎、叶和四轮花器官中均有表达.花瓣中的表达量最高,叶片中表达量最低.
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