柳树种质资源遗传多样性和亲缘关系的CE-AFLP分析

摘要

利用毛细管电泳-AFLP技术,采用EcoR Ⅰ+3/Mse Ⅰ+3和Pst Ⅰ+2/Mse Ⅰ+3引物组合,对从国内外搜集的37份柳树种质资源进行遗传多样性和亲缘关系分析.11对引物共扩增出1 580条条带,多态性条带数为1 399条,多态率在80.65％ ～ 94.67％之间,表明柳树种质间的遗传多样性较高.采用NTSYS-pc 2.10e软件对37份种质进行聚类分析和主成分分析,在相似系数0.63时,37份种质分为2大类,一类是乔木柳,一类除小乔木康定柳外,其余都为灌木柳.聚类分析和主成分分析结果与传统形态分类基本一致,但筐柳组中簸箕柳P295和沙柳基因组水平上差异较大,P295与银芽柳亲缘关系较近.研究结果为柳树分子生物学分类、种质资源保存与利用及品种改良等研究提供理论依据.%Genetic diversity and genetic relationship of 37 Salix germplasms from domestic and foreign were analysed using the CE-AFLP (capillary electrophoresis-amplified fragment length polymorphism)technique.The primer combinations of EcoRⅠ + 3/MseⅠ + 3 and PstⅠ + 2/MseⅠ + 3 were used in this study.The result indicated that the 11 primer pairs were used to generate 1 580 fragments,of which 1 399 fragments were polymorphic.The polymorphism rate varied from 80.65％ to 94.67％,showing a high genetic diversity among Salix.NTSYS-pc 2,10e software was used for cluster analysis and principal component analysis.The 37 germplasms were divided into two major categories with the similarity coefficient of 0.63,and one belonging to arbor Salix and others to the shrubs except for S.paraplesia.Cluster analysis and principal component analysis were generally consistent with traditional taxonomy.However,there was obvious difference in the genome level between S.suchowensis and S.psammophi in the section Helix.This study on the genetic diversity and genetic relationship of some Salix germplasms at the DNA level would provide a theoretical basis for Salix molecular classification,germplasm resources conservation and utilization,and variety improvement.