Genetic Relationship among Germplasm Resources of Buckwheat in Northwestern Sichuan Using RAPD and Isozyme Marker

摘要

The genetic relationship of ten representative species of Buckwheat collected in north western of Sichuan was analyzed by using random amplified polymorphic DNA(RAPD)and Isozyme marker. The obtained results showed that a total of 388 bands were detected in 14 random primer combinations selected from 40 combinations among ten species．Of these bands, 352(90．7%)were polymorphic．Fifteen peroxjdaSe enzyme bands(POD)could differentiate ten species, while 10 esterase isozyme(EST)bands distinguish these species．Cluster analysis divided them into four groups, 1)Esmtice (A'ba), F.gracilipes var．odontopterum(A'ba);2)Fagopyrum tataricum;3)Egracilipes, Var．odontopterum, F．gracilipes and 4)cymosum(Xichang), F．esculentum, F．cymosum(A'ba), F．cymosum(Ya'an)．