Drought-induced proteins,which are induced or up-regulated by water deficient stress,play an important role in drought resistance process of plants. In this research,full length cDNA of PwWDS1 ( GenBank accession number KJ526353),a drought induced gene,was obtained by RACE PCR method based on the full length cDNA library of Picea wilsonii. Several software and tools such as Expasy,DNAMAN,ClustalX and MEGA were used to analyze the physical and chemical properties of PwWDS1. Then expression pattern of PwWDS1 was identified by RT-qPCR and semi-quantitative RT-PCR under drought,salt stress,abscisic acid ( ABA) and ethrel treatments. We found that the full length cDNA of PwWDS1 was 1 077 bp encoding a protein which consists of 207 amino acids residues. The theoretical molecular weight of PwWDS1 was 22. 76 kDa with isoeletric point of 5. 02. Sequence analysis indicated that C terminal of PwWDS1 had a conserved ABA/WDS domain and N terminal had a strong hydrophilic property. RT-qPCR and semi-quantitative RT-PCR showed that PwWDS1 was mainly expressed in needles of P. wilsonii and the expression level of PwWDS1 increased with the growth of cotyledon during seed germination. The PwWDS1 expression was up-regulated by drought,salt stress,ethrel and ABA treatments. PwWDS1 was expressed highly in 6 h under drought and ABA treatments. Under salt and ethrel treatments,PwWDS1 was expressed most in 12 h. These results indicated that PwWDS1 would play a role in response to signals of abiotic stresses and plant hormones in P. wilsonii.
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