长果种黄麻SRAP标记遗传连锁图谱的构建及3个质量性状基因定位

摘要

[目的]构建黄麻遗传连锁图谱,定位质量性状基因,为今后有关黄麻基因组结构、重要农艺性状QTL定位、分子标记辅助育种和基因克隆等研究工作奠定基础.[方法]以甜麻(黄麻野生种)和宽叶长果(黄麻栽培品种)为杂交亲本,构建了187个F2单株作为作图群体,利用513对SRAP引物进行遗传图谱构建,并对3个质量性状基因(托叶色、叶柄色、叶缘色)进行了定位.[结果]122个SRAP多态性标记位点和这3个形态学标记被定位在该图谱上,初步构建的长果种黄麻遗传连锁图谱全长2 231.9 cM,包含10个连锁群,每个连锁群有2-38个标记位点,2个标记间平均间距为17.86 cM.[结论]该图谱上的标记位点均匀分布在10个连锁群上,没有出现标记位点聚集的现象,表明SRAP标记十分适合黄麻遗传图谱的构建.%[Objective] Jute (Corchorus olitorius L.) is one of the most economically important crops for fiber production. The present study was aimed to construct a molecular linkage map of jute and locate important qualitative traits, which will provide essential tools for investigating genomic structure and evolution, facilitating QTL mapping, marker-assisted selection and map-based cloning of important gene. [Method] In this study, a population of F2 generation including 187 individual plants was derived from the cross between Corchorus aestuans L. (wild species) and wild leaf jute (cultivated species). A total of 513 sequence-related amplified polymorphisms (SRAP) primer pairs were used in constructing the molecular genetic linkage map and mapping 3 qualitative traits including color of stipule, color of stipe, and color of leaf margin. [Result] This genetic linkage map containing 10 linkage groups (LGs) was constructed using 122 SRAP loci and these 3 morphological markers, the map covered 2 231.9 cM with the average marker interval of 17.86 cM, every linkage group included 2-38 markers. [Conclusion] All the markers are distributed evenly among the linkage groups without clustering of loci. SRAP markers may be a better type of markers in map construction.