[目的]寻找与番鸭就巢性状相关的表达基因,了解其就巢调控的分子机理.[方法]采用cDNA-AFLP(cDNA-amplified fragment length polymorphism)技术分析遗传背景相近的RF系白羽番鸭就巢个体和非就巢个体脑垂体基因表达差异.[结果]筛选到与就巢行为相关差异表达片段82条,选取其中15条进行测序.将测序结果在GenBank数据库进行BLAST比较和分析,发现8个片段(53.3％)与已知基因具有较高同源性,涉及与繁殖性能紧密相关的下丘脑-垂体-性腺轴(hypothalamic-pituitary-gonadal,HPG)上的功能基因,如GnRH基因、FSH基因,同时也分离到位于GH/IGF轴上的功能基因,即GH基因；以及在功能上属于信号传导、转录调控、代谢的调节基因,如超氧化物歧化酶基因、角蛋白关联蛋白基因、硫氰酸酶释放硫基转移酶基因等.2个片段与未知功能的基因有同源性,5个与数据库中现有的基因没有明显的相似性.随机选择3个片段进行RT-PCR验证,检测片段的表达模式与cDNA-AFLP结果一致.[结论]揭示番鸭不同就巢性状基因表达调控模式,进一步阐明番鸭就巢的分子机理.%[Objective] The objective of this experiment is to identify differentially expressed genes of Muscovy Duck broodiness. [ Method ] cDNA-AFLP approach was employed to analyze genes differentially expressed in broodiness and non-broodiness Muscovy Duck individuals derived from a single RF white feathers line. [Result] There were 82 differentially expressed bands associated with broodiness behaviors, 15 bands of them were subject to sequence analysis. Database searches indicate that 8 cDNA fragments show high homology to known genes, which include some function genes in hypothalamic-pituitary-gonadal axis regulating reproduction, such as GnRH gene and FSH gene. Meanwhile genes were also found in GH-IGF axis, such as GH gene. Other genes participated in signal transduction, transcription, metabolism biological processes, such as superoxide dismutase, keratin related protein, thiocyanate enzyme release S-transferase enzymes etc. Two cDNA fragments show homology to unknown function genes, 5 cDNA fragments show no detectable homology to know genes, suggesting that they probably represent novel genes. Three bands selected randomly from them were confirmed by RT-PCR and that result was consistent with the results of cDNA-AFLP. [Conclusion] The results of this paper uncovered the regulation mode of gene express variation of different Muscovy Duck broodiness traits, and thus further providing the molecular mechanism of broodiness of Muscovy Duck.
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