基于元分析的大豆成熟期单片段代换系鉴定与QTL定位

摘要

[目的]大豆成熟期是由多基因控制的数量性状，是影响大豆产量和适应性的重要性状。研究大豆成熟期单片段代换系遗传规律，鉴定分析大豆成熟期的主效QTL。[方法]以大豆红丰11为受体和回交亲本，以15个国内外大豆核心种质为供体亲本，构建回交导入系群体，基于元分析的大豆成熟期（R8）“真实QTL”SSR标记进行单片段代换系鉴定，利用“图示基因型法”计算导入片段和代换作图法鉴定大豆成熟期 QTL，用单标记法鉴定成熟期重要QTL。[结果]在C2、L连锁群上检测到16种导入片段，C2连锁群检测到7种导入片段，导入片段总长度为9.8 cM。L连锁群检测到9种导入片段，导入片段总长度为37.212 cM；在C2和L连锁群上共检测出8个成熟期QTL，根据前人的研究，在L连锁群上有2个QTL即Sat_010、Satt156是E4/e4的特异SSR标记；在8个成熟期 QTL 中用单标记法鉴定了5个有关大豆成熟期重要 SSR 标记 Sat_238、Satt460、Sct_010、Satt166、Sat_113；确定了单片段Satt460缩短大豆生育期，单片段Sat_238、Sct_010、Sat_113延迟大豆生育期。[结论]基于元分析的成熟期2个导入位点C2、L连锁群上检测到16种单片段，用代换作图法共检测出8个成熟期QTL，用单标记法鉴定了5个有关大豆成熟期重要SSR标记Sat_238、Satt460、Sct_010、Satt166、Sat_113。确定单片段Satt460与缩短大豆生育期有关，单片段Sat_238、Sct_010、Sat_113与延迟大豆生育期有关。%[Objective]Soybean maturity is a quantitative trait controlled by multiple genes, which is a key trait affecting soybean yield and adaptability. The heredity of soybean maturity with single segment substitution lines (SSSLs) was studied and the main effect QTLs of soybean maturity were identified. [Method] Fifteen sets of backcross introgression lines were constructed by using Hongfeng 11 as recurrent parent and 15 soybean varieties as the donor parent, respectively. Based on the results of meta-analysis of soybean maturity (R8), the SSR markers from real QTL interval were used to identify SSSLs. Using the method of figure genotype calculated the length of substitution fragment, using the statistical software SPSS analyzed the main effect QTLs of soybean maturity. [Result]Sixteen substitution fragments were detected on linkage group C2 and L. Among them, 7 substitution fragments were detected on linkage group C2, substitution fragment length was 9.8cM in total. Nine substitution fragments were detected on linkage group L, and the substitution fragment length was 37.212 cM in total. Previous researches show that Sat_010 and Satt156 are the specific markers for E4/e4 on linkage group L, and 5 QTLs(Sat_238, Satt460, Sct_010, Satt166, and Sat_113 )were identified on the maturity in one-way. Single fragment Satt460 could shorten the growth period of soybean, and single fragments Sat_238, Sct_010 and Sat_113 could delay soybean growth stages. Sat_238 Satt460, Sct_010, Satt166, and Sat_113 were identified to be the important SSR markers on soybean maturity. [Conclusion]Five markers from meta-analysis were identified as the important markers on soybean maturity. The single fragment Satt460 could shorten the soybean growth period, single segments Sat_238, Sct_010, and Sat_113 could delay soybean growth period.