HPLC法检测细胞培养上清液中前列腺素E2的含量

摘要

建立了检测巨噬细胞培养上清液前列腺素E2（PGE2）含量的高效液相色谱法,为检测体外细胞培养液PGE2提供快速定量的方法。色谱条件为：Ultimate XB-C18反相柱（250mm×4.6mm,5μm）;以乙腈和0.02mol/L磷酸二氢钾水溶液（60∶40,v/v）作为流动相;流量为1mL/min;检测波长为196nm。结果表明：该方法标准曲线良好,线性范围为0.5625～90μg/mL,相关系数为R2=0.999,出峰时间为3.988min,最低检测限为0.1448μg/mL,平均回收率为100.71%。本方法简单快速,适用于样品数目较多的PGE2的快速定量检测。%A HPLC method was developed for determination of PGE2 contents in supernatants of RAW264.7 murine micropages.The samples were separated on Ultimate XB-C18 column（250mm×4.6mm,5μm）,eluted with mixture of acetonitrile and potassium dihydrogen phosphate solution 0.02mol/L（60：40,v/v） at 1mL/min,and detected at 196nm.Results showed that the calibration curves of PGE2 had good linearity.The developed method exhibited good linearity over the range from 0.5625 to 90μg/mL with a correlation coefficient of 0.999.The minimum detection limit was 0.1448μg/mL and the peak time was 3.988min.The average recovery rate for PGE2 was 100.71%.This method was simple and fast,which was suitable for determination of PGE2 with a large sample.