重组大肠杆菌利用不同培养基发酵产琥珀酸的研究

摘要

微生物发酵法生产琥珀酸具有广阔的应用前景,实验针对实验室构建的工程菌Escherichia coli WS100(△ldhA、△adhE、△pflB、△poxB、△ackA)在不同培养基中进行厌氧发酵,考察其发酵特性.分别采用NBS培养基和玉米浆培养基,在含4L发酵液的发酵罐中进行补料-分批发酵.发酵83h后,Escherichia coli WS100在玉米浆培养基中消耗葡萄糖96.17g/L,积累琥珀酸63.45g/L,生产强度和质量收率分别为0.76g/(L·h)和65.98％；Escherichia coli WS100在NBS培养基中消耗葡萄糖ll0.30g/L,琥珀酸的产量达到84.98g/L,生产强度和质量收率分别为1.02g/(L·h)和77.04％.实验结果表明,Escherichia coli WS100在玉米浆培养基和NBS培养基中发酵均有较高的琥珀酸产量,其中在NBS培养基中琥珀酸的产量、生产强度和质量收率均比在玉米浆培养基中高.%The production of succinic acid by bacterial fermentation has a great prospect for industrial application. In this study,the fermentative productions of succinic acid by recombinant Escherichia coli WS100 (△ldhA, △adhE,△pflB,△poxB,△ackA) with different media were investigated. 4L NBS medium and corn steep liquor medium were utilized respectively for fed batch fermentations with E.coli WS100 in fermenters. After 83h of fermentation,96.17g/L glucose was consumed in corn steep liquor medium,with the production of 63.45g/L succinate. The productivity and yield reached 0.76g/(L·h) and 65.98%,respectively. In NBS medium, 110.30g/L glucose was consumed with the production of 84.98g/L succinate. The productivity and yield reached 1.02g/(L·h) and 77.04%, respectively. The results indicated fine succinate fermentative production was by WS100 in both media, and with higher productive amount, productivity and yield in NBS medium.