纳米硒桔梗多糖复合物对CCl4致小鼠肝损伤的保护作用

摘要

Using platycodon Polysaccharide and Na2SeO3 as raw materials,the complex containing nano-selenium and platycodon polysaccharide was prepared by reduction method by virtue of vitamin C as reductant.The mice model with injury liver induced by CC]4 was founded.By detection of biochemical indicator of blood serum,malondialdehyde(MDA) content and glutathione peroxidase(GSH-Px) activity in liver tissue,the liver index was calculated.According to the histopathological changes in liver,the protective effect of nano-selenium/platycodon polysaccharide complex on liver injury induced by CCl4 in mice was evaluated.The results indicated that alanine aminotransferase (ALT),aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the serum of mice were lower than those of the control group.Total protein (TP) and albumin (ALB) levels was observed to increase in varying degrees.At the same time,The activity of glutathione peroxidase(GSH-Px) in the tissues significantly (p ＜ 0.05) increased,while the content of malondialdehyde (MDA) decreased significantly (p ＜ 0.05).For medication group,it was shown by pathological sections that SePP can protect liver cells from damage by CCl4 chemicals.It was confirmed that the complex containing nano-selenium and platycodon presented stronger protective effect on liver injury induced by CCl4 in mice than that of platycodon polysaccharide.h paves a way to the development of new selenium containing liver protecting drugs.%以桔梗多糖和Na2SeO3为原料,采用维生素C还原法制备纳米硒桔梗多糖复合物.建立CCl4小鼠化学损伤肝模型,通过检测小鼠血清生化指标和肝组织中丙二醛(MDA)的含量和谷胱甘肽过氧化物酶(GSH-Px)的活性,计算肝脏指数,观察肝组织病理学变化;评价纳米硒桔梗多糖复合物对CCl4致小鼠肝损伤的保护作用.结果表明:各给药组与模型组相比,小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)和碱性磷酸酶(ALP)水平有不同程度的降低;总蛋白(TP)和白蛋白(ALB)的含量有不同程度升高.同时,组织中GSH-Px活力显著(p＜0.05)升高,MDA含量显著(p＜0.05)降低.病理切片显示,纳米硒桔梗多糖复合物各给药组可不同程度地保护肝脏细胞避免CCl4的损害.证实纳米硒桔梗多糖复合物对CCl4致小鼠肝损伤有一定的保护作用且强于桔梗多糖,为开发新型的含硒保肝药物提供了一定的科学依据.