γ－聚谷氨酸产生菌发酵培养基的响应面法优化研究

摘要

γ-polyglutamic acid was produced by fermentation with bacillus natto screened from natto. Op-timization of the fermentation medium by response surface method on the basis of single factor experiments was studied. Results revealed that the optimal medium constituents were as follows:sucrose 43. 92 g/L, soy peptone 7. 00 g/L,sodium glutamate 46. 32 g/L. The production of γ-PGA increased from 7. 253 g/L to 11. 794 g/L.%利用从纳豆中筛选得到的一株纳豆芽孢杆菌发酵生产γ－聚谷氨酸（γ－PGA）。在单因素优化实验的基础上，通过响应面法对发酵培养基进行优化，得到最佳培养基配方为蔗糖43．92 g／L、大豆蛋白胨7．00 g／L、谷氨酸钠46．32 g／L，γ－PGA产量由原来的7．253 g／L提高到11．794 g／L。