土家药物红刺老苞水提物对类风湿关节炎成纤维滑膜细胞增殖的影响

摘要

Objective:To investigate the effects of Aralia echinocaulis Hand.Mazz.,a medicine of Tujia minority,on the proliferation of fibroblast-like synoviocytes(FLSs)of rheumatoid arthritis. Methods:Collected synovial tissue specimens from 1 patients with rheumatoid arthritis and cultured them into FLSs in vitro.After 48 hours' process of FLSs with Aralia echinocaulis Hand.Mazz. water extract of different concentrations,the MTT method was used to detect the level of cell proliferation and the flow cytometry was used to detect the apoptosis.Results:The cell proliferation was inhibited(P < 0.01) after the FLSs were processed with Aralia echinocaulis Hand.Mazz. aqueous extract,and the inhibitory rate of cell proliferation was positively correlated with the drug content.The apoptosis rate of FLSs sincreased(P<0.01).Conclusion:Aralia echinocaulis Hand.Mazz. can inhibit abnormal proliferation of synovial cells into ifbrous synovial cells.%目的：探讨土家族药物红刺老苞根皮水提物对类风湿关节炎成纤维滑膜细胞异常增殖的影响。方法：收集1例类风湿关节炎患者的滑膜组织标本，体外培养成纤维滑膜细胞，不同浓度的红刺老苞根皮水提物处理成纤维滑膜细胞48 h后，MTT法检测成其增殖水平，流式细胞术检测其凋亡影响。结果：红刺老苞水提物处理成纤维滑膜细胞后，细胞增殖受到抑制（P<0.01），并且细胞增殖抑制率与药物含量正相关；而且还能增加成纤维滑膜细胞的细胞凋亡率（P<0.01）。结论：土家族药物红刺老苞能够抑制成纤维滑膜细胞的异常增殖。