抗磷脂综合征中抗TFPI抗体表达及临床意义

摘要

Objective:To study the expression of anti-TFPI antibody in antiphospholipid syndrome and its clinical significance.Methods:The anticardiolipin antibody and anti-β2GPI antibody were determined with the ELISA method after collecting plasma/serum of 26 patients with primary antiphospholipid syndrome and 24 antiphospholipid antibody positive asymptomatic patients.At the same time,determined the lupus anticoagulant,recorded the patients’ clinical data,and determined anti-TFPI antibody(IgG+IgM) with the ELISA method.In addition,serum of 40 healthy people taking medical examination was collected as reference value of anti-TFPI antibody.Results:The expression rate of anti-TFPI antibody in patients with antiphospholipid syndrome was 30.77%,significantly higher than that of the normal control(P < 0.05).The incidence of thrombosis of the positive group of anti-TFPI antibody signiifcantly increased compared with that of the negative group(P < 0.05).There was no significant positive correlation between the anti-β2GPI antibody and the anti-TFPI antibody(r = 0.15).Conclusion:The higher expression of the anti-TFPI antibody in patients with antiphospholipid antibodies showed that the anti-TFPI antibodies might have some effects on thrombosis.At the same time,the antibody would help to diagnose antiphospholipid syndrome in some potential patients.%目的：研究国内抗磷脂综合征患者体内抗组织因子途径抑制物（TFPI）抗体的表达情况及其临床意义。方法：通过收集26例原发性抗磷脂综合征患者及24例抗磷脂抗体阳性无症状者的血浆/血清，ELISA法测定其抗心磷脂抗体、抗β2GPI抗体，同时测定狼疮抗凝物并记录患者的临床资料， ELISA法测定抗TFPI抗体（IgG+IgM）。另外随机收集40名健康体检者血清，作为抗TFPI抗体参考值。结果：抗磷脂综合征患者抗TFPI抗体表达率（30.77%）较正常对照组明显升高（P<0.05），抗TFPI抗体阳性组血栓发生率较阴性组明显升高（P<0.05），抗β2GPI抗体与抗TFPI抗体无明显正相关（r=0.15）。结论：抗磷脂抗体阳性患者体内抗TFPI抗体高表达，抗TFPI抗体可能对血栓形成具有某种促进作用，同时该抗体将有助于一些潜在的抗磷脂综合征患者的诊断。