莶草对尿酸钠引起痛风性关节炎IL-1β、TNF-α、NF-κB表达的影响

摘要

Objective:To research the mechanism of action of Xixiancao[Herba Siegesbeckiae] to the cell inlfammatory factor of gouty arthritis induced by sodium urate.Methods: 108 Wistar male rats of clean grade 10-13 weeks were randomly divided into blank control group,model control group,Xixiancao high dose group,Xixiancao middle dose group group,Xixiancao low dose group and colchicine group,18 rats in each group. Except for the blank control group,the other groups by gouty arthritis rat model induced by sodium urate.The models were given different dosage of Xixiancao and colchicine by intragastric administration,observing their pathological changes of articular tissue and measuring the expression of IL-1β,TNF-α and NF-κB.Results:The rats in the model control group had knee joint swelling obviously and couldn’t move with force.The level of IL-1β, TNF-α and NF-κB in the leachate form the swollen joints signiifcantly increased.There were synovial cell hyper-plasia,inlfammatory cell inifltration,arrangement in disorder,ifbroblast proliferation,tissue necrosis and inlfammato-ry exudation.The therapeutic effect of the high and middle dose groups of Xixiancao was obvious and red swelling weakened.The phlogocyte inifltration in sur-rounding soft tissue of the high dose group of Xixiancao was not obvious and IL-1β, TNF-α and NF-κB in the leachate form the swollen joints signiifcantly decreased.Conclusion:By the expression of IL-1β,TNF-α and NF-κB,Xixiancao could effectively inhibit the local inlfammatory reaction of gouty arthritis.%目的：研究豨莶草对尿酸钠引起的痛风性关节炎细胞炎性因子的作用机制。方法：选取健康清洁级10～13周龄雄性Wistar大鼠108只，随机分为空白对照组、模型对照组、莶草高剂量组、莶草中剂量组、莶草低剂量组、秋水仙碱组，每组18只。除空白对照组外，其余各组均通过尿酸钠致大鼠痛风性关节炎模型，用莶草不同剂量和秋水仙碱对模型大鼠灌胃给药。观察各组大鼠关节组织的病理学变化和测定白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、核转录因子-κB（NF-κB）的表达。结果：模型对照组大鼠膝关节红肿明显且不敢发力，肿胀关节浸出液中IL-1β、TNF-α、NF-κB水平显著升高，滑膜细胞增生、炎性细胞浸润，排列紊乱，成纤维细胞增生，组织坏死并且炎性渗出。莶草高、中剂量组治疗效果明显，红肿减弱，莶草高剂量组周围软组织中炎细胞浸润并不明显，肿胀关节浸出液中IL-1β、TNF-α、NF-κB均显著降低。结论：莶草通过抑制IL-1β、TNF-α、NF-κB表达，可有效抑制痛风性关节炎局部的炎症反应。