视网膜前膜内相关炎性细胞因子的免疫组化研究

摘要

Objective　To investigate the expression and role of inflammatory cytokines like interleukin-1β(IL-1β)、interleukin-6(IL-6)、interleukin-8(IL-8) and tumor necrosis factor-alpha(TNF-α)in epiretinal membranes(ERM)of eyes with proliferative vitreoretinopathy(PVR).Methods　Nineteen epiretinal membranes were obtained from eyes undergoing vitrectomy for retinal detachment complicated with PVR and observed by immunohistochemical methods.Results　Expression of IL-1β、IL-6、IL-8 and TNF-α were observed in 9、12、11and 15 membranes respectively, with positive staining mostly in the extracellular matrix of epiretinal membranes. Only one membrane showed positive to IL-6 intracellularly, expression for all the cytokines simultaneously in 4 membranes.Conclusion　The findings indicate that cytokine-mediated pathways of inflammation are involved in the pathogenesis of PVR.%目的研究增生性玻璃体视网膜病变(proliferative vitreoretinopathy, PVR)的视网膜前膜(epiretinal membranes, ERM)中炎性细胞因子白细胞介素-1β(interleukin-1β，IL-1β)、白细胞介素-6(interleukin-6，IL-6)、白细胞介素-8(interleukin-8，IL-8)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达及其作用。方法用免疫组织化学方法对19例复杂性视网膜脱离行玻璃体切割术时剥离的视网膜前膜进行观察。结果 IL-1β、IL-6、IL-8和TNF-α分别在9、12、11和15例视网膜前膜中表达，尤其以细胞外基质中明显，4种细胞因子在4例膜中同时表达，仅有1例膜中细胞内表达IL-6。结论增生性玻璃体视网膜病变的发病机理中有细胞因子介导的炎症途径参与。