60Co γ射线对人离体外周血 CDKN1A 基因表达水平的影响

摘要

目的：探讨60 Co γ射线对人离体外周血 CDKN1A 基因表达水平的影响。方法收集3名健康人周围血，采用60 Co γ射线照射，照射剂量率为0．313 Gy/min 。提取血液总 RNA ，用实时荧光定量 PCR 方法检测 CDKN1A 基因的表达水平。结果0～2 Gy 剂量范围内，CDKN1A 的 mRNA 相对表达水平随照射剂量增加而呈上调趋势，拟合的回归方程为：Y ＝0．8465＋1．1015D（R ＝0．93，p ＜0．01），Y ＝0．6273＋3．0883D －0．7485D2（R ＝0．96，p ＜0．05）。结论电离辐射可诱发 CDKN1A 基因相对表达水平发生改变，并且随照射剂量增加而增加，其中直线方程的稳定性还有待进一步验证。%To explore the effect of ionizing radiation on CDKN1A gene expression in human peripheral blood , blood samples were exposed to 60 Co γ-rays at different doses .The resulted CDKN1A mRNA expression change was observed by real time PCR .For a dose of 0 ~ 2 Gy ,the relationship between the CDKN1A-dose represents certain line correlation .A linear regression equation(Y = 0 .8465 + 1 .1015D ,R = 0 .93 ,p < 0 .01) and cur-vilinear regression equation (Y = 0 .6273 + 3 .0883D - 0 .7485D 2 ,R = 0 .96 ,p < 0 .05) has been obtained using regression analysis methods .γ-ray can induced CDKN1A gene expression changed and increase dose-de-pendently ,but the stability of regression equation should be tested further .