rhEPO 对缺糖缺氧大鼠星形胶质细胞GLT-1和 GLAST 表达的影响

摘要

为了研究重组人促红细胞生成素（rhEPO）对缺糖缺氧（OGD）培养大鼠星形胶质细胞 GLT-1和 GLAST 表达的影响，将缺糖缺氧培养星形胶质细胞分成不同浓度 rhEPO 处理组：0、20、100 U／mL，不同浓度 rhEPO 与星形胶质细胞在缺氧缺糖条件下培养6 h，用 RT-PCR 测定 GLT-1和 GLAST 的 mRNA 表达变化，免疫印迹技术测定 GLT-1和 GLAST 蛋白的表达变化。20、100 U／mL rhEPO 星形胶质细胞 GLT-1的 mRNA 和蛋白质水平较 OGD 对照组明显升高（P ＜0．05），GLAST 的 mRNA 和蛋白质水平变化不明显（P ＞0．05）。GLT-1水平可能与 rhEPO 对缺糖缺氧培养大鼠星形胶质细胞的保护作用有关。%In order to study effects of rhEPO on the expressions of GLT-1 and GLAST in rat astrocytes cul-tured by oxygen-glucose deprivation,the astrocytes of rats cultured by oxygen-glucose deprivation were di-vided into three groups with different concentrations of rhEPO 0,20,100 U/mL and cultured for 6 hours by hypoxia-glucose deprivation.The real-time PCR and Western blot were used to detect the changes of mRNA and protein expressions of GLT-1 and GLAST,respectively.In comparison with OGD control,mR-NA and protein levels of GLT-1 were found to be increased in the groups of 20 and 100 U/mL rhEPO (P <0.05),but the changes of GLAST were not obvious(P >0.05).The changes of GLT-1 may be related to protective effects of rhEPO on astrocytes of rats cultured by oxygen-glucose deprivation.