Construction of human and mouse brain cDNA libraries and isolation of full-length cDNAs

摘要

＜正＞ cDNA libraries from aborted human 3-month fetal brain, adult rat and mouse brain were constructed by using a XZAP express cDNA library construction kit. Low molecular weight fragments of the second strand cDNAs were removed by flowing through the Sepharose CL-4B column and the fractionated long, middle, short fragments and the combined fragments were respectively inserted into clone vectors to construct the cDNA libraries of the brain of human 3-month fetus. The 5’ ends of 1200 clones from each of human fetal brain cDNA libraries were sequenced. A total of 894 ESTs were obtained and some full-length clones were sequenced. By analyzing the sequences, 12 novel full-length cDNAs were obtained.