肥大细胞及类胰蛋白酶在人甲状腺肿瘤组织中的表达及其与微血管密度的关系

摘要

Objective: To investigate the correlation between the expression of mast cell (MC) as well as tryptase and microvessel density (MVD) in in human thyroid tumor. Methods: Compare with and study the expression of MC, tryptase and CD31 which were examined by histochemistry and PV immunohistochemistry in 116 cases of thyroid carcinoma, 56 cases of thyroid adenoma, 10 cases of normal thyroid tissues; and analyzed the correlation of the MC, tryptase expressions and the MVD. Results: The number of MC and the expression of tryptase in thyroid tumor tissues were higher than that in normal thyroid, and the number of MC and the expression of tryptase was correlated with the type of tumor(P<0.01). Spearman rank correlation analysis showed that the number of MC was positively correlated with the MVD in the thyroid tissues (r=0.900,r=0.636, P<0.05). Conclusions: The MC and tryptase may promote to the angiogenesis of thyroid tumor, and so may promote invasion and metastasis of the thyroid tumor.%目的:探讨肥大细胞(mast cell,MC)及类胰蛋白酶(tryptase)与甲状腺肿瘤微血管密度(microvessel density,MVD)的相关性及其对甲状腺癌发生发展的影响.方法:采用甲苯胺蓝组织化学染色和PV免疫组织化学染色对116例甲状腺癌、56例甲状腺腺瘤和10例正常甲状腺组织中MC和tryptase及其CD31的表达进行了检测；对MC和tryptase与MVD的关系进行了统计学分析.结果:甲状腺肿瘤中MC的数量和tryptase阳性表达高于正常甲状腺,而且与肿瘤的类型有关(P＜0.01)；Spearman等级相关分析显示各组甲状腺组织MC数量和tryptase表达与MVD呈正相关(r=0.900,r=0.636,P＜0.05).结论:MC及其分泌的tryptase有促进血管新生的作用,因而可促进甲状腺肿瘤的浸润和转移.