脑深部电刺激自身给药大鼠伏隔核区△FosB的表达

摘要

Objective:To take the implementation of the bilateral NAc-DBS surgery on morphine-addiction rats.Then we analyzed the change of △FosB expression in NAc which providing molecular evidence for the NAc-DBS in the treatment of drug dependence.Methods:18 adult rats weighing 240-260 g were divided into three groups,which including the DBS group (n=6,administration of surgery,morphine and DBS),the sham DBS group (n=6,administration of surgery and morphine) and the control group (n=6,administration of physiological saline).Results:The bilateral NAc DBS attenuated morphine-induced reinstatement of drug seeking.The following immunohistochemistry measurements revealed that the behavioral consequences should be associated with a significant reduction in the expression of △FosB in the NAc.Conclusion:These findings have indicated that the NAc DBS could be an effective and safe therapeutic option for preventing relapse to morphine addiction.%目的:对吗啡依赖大鼠实施双侧伏隔核脑深部电刺激(NAc-DBS),分析NAc区△FosB的表达变化,为NAc-DBS治疗药物依赖提供分子生物学证据.方法:18只大鼠随机分为三组,包括DBS组(n=6,实施颈静脉插管和电板植入手术,吗啡给药,DBS),Sham组(n=6,实施颈静脉插管和电极植入手术,吗啡给药),Control组(n=6,实施颈静脉插管和电极植入手术,给予生理盐水),观察DBS组大鼠在高频电烈激期(160 Hz,1 h/d,7d)的觅药行为变化,然后进行取脑,切片,免疫组化染色,观察伏隔核区△FosB的表达.结果:成瘾大鼠在高频电刺激期,觅药行为明显减少；免疫组化染色后观察到DBS组大鼠NAc区△FosB的表达相对于Sham和Control组明显减少.结论:双侧NAc-DBS抑制吗啡成瘾大鼠的觅药行为以及NAc区△FosB的表达,证实△FosB可能是慢性成瘾转换机制的关键分子的观点.