Toxicity Studies of 2-Hydroxy-4-Methoxybenzophenone (CAS No. 131-57-7)Administered Topically and in Dosed Feed to F344/N Rats and B6C3F1 Mice

摘要

Toxicity studies of 2-hydroxy-4-methoxybenzophenone (HMB) were performed in ratsand mice by administering the compound in feed and topically. Assessments included hematology, clinical chemistry, urinalysis, reproductive toxicity, and histopathologic evaluations. HMB produced generally similar effects following topical and oral administration to rats and mice. Consistent findings included decreases in epididymal sperm density, lengthened estrous cycle, and increased liver and kidney weights. Mice in the dosed feed studies exhibited microscopic changes in the kidneys, comprising tubular dilation with eosinophilic protein casts. Dilation, tubular regeneration, papillary degeneration, and inflammation were noted in the kidneys of rats; liver lesions consisting of an apparently reversible hepatocyte cytoplasmic vacuolization occurred in both rats and mice. The genetic toxicity of HMB also was evaluated in mutagenicity studies with Salmonella typhimurium, in cytogenetic studies with Chinese hamster ovary (CHO) cells, and by evaluation of micronucleated erythrocytes in peripheral blood smears from mice in the 13-week studies. HMB was weakly mutagenic in Salmonella with metabolic activation, and induced sister-chromatid exchanges and chromosomal aberrations in CHO cells in the presence of a metabolic activation system. There were no increase in the frequency of micronucleated erythrocytes in the blood of mice receiving HMB.