低浓度万古霉素复合PMMA骨水泥的细胞毒性研究

摘要

目的:研究低浓度万古霉素(1％)复合聚甲基丙烯酸甲酯PMMA骨水泥对SD仔鼠原代成骨细胞的增殖以及凋亡的影响.方法:采用分离SD仔鼠颅骨与胰酶消化法获取SD仔鼠原代成骨细胞,通过细胞爬片碱性磷酸酶染色对原代成骨细胞进行鉴定;利用CCK-8法检测低浓度万古霉素复合PMMA及纯PMMA骨水泥浸提液对SD大鼠原代成骨细胞的增殖影响、流式细胞仪检测万古霉素复合PMMA及纯PMMA骨水泥浸提液对对SD大鼠原代成骨细胞的凋亡影响.结果:SD大鼠原代成骨细胞在低浓度万古霉素复合PMMA骨水泥浸提液中第1、2、3天的增殖更明显(P＜0.05),在纯PMMA骨水泥浸提液中第1、5天凋亡显著增加(P＜0.05).结论:低浓度万古霉素复合PMMA骨水泥相比PMMA骨水泥具有较低的细胞毒性,在临床应用于骨缺损伴局部骨感染的治疗方面具有一定的优势.%Objective:To study the cytotoxicity of vancomycin (low concentration,l％)/polymethyl methacrjlate (PMMA) cements on SD rat-derived primary osteoblasts.Methods:Primary osteoblasts were obtained by the calvarias trypsin-digestion method,which were isolated from newborn Sprague-Dawley rats.To identify the primary osteoblasts,alkaline phosphatase staining was used.The activity of cell proliferation in vancomycin (low concentration)/polymethyl methacrylate (PMMA) and polymethyl methacrylate (PMMA) cements extract were assessed by CCK-8 assay.Apoptosis of osteoblasts induced by vancomycin (low concentration)/ polymethyl methacrylate (PMMA) and polymethyl methacrylate (PMMA) cements extract was observed by flow cytometry.Results:The activity of SD rat rat-derived osteoblasts proliferation in the vancomycin (low concentration)/polymethyl methacrylate (PMMA)cement extracts was significantly more than that in the group of PMMA cement extracts on day 1,2,3 (P ＜ 0.05).The apoptotic rate of primary osteoblasts in extracts from the vancomycin (low concentration)/PMMA cements was significantly lower than that in extract from the PMMA cement on day 1 and 5 (P ＜ 0.05).Conclusion:Compared with PMMA cements,the vancomycin (low concentration)/PMMA cements has a good cytocompatibility with primary osteoblasts.It will be useful inbone defect with infection repair.