目的评价氢氧化铝[ Al（ OH）3]佐剂对铜绿假单胞菌11型O-特异性多糖与破伤风类毒素结合物免疫原性和免疫保护性的影响。方法用热酚水法提取铜绿假单胞菌11型（IATS 11）菌株的脂多糖（LPS），去除类脂A并纯化O-特异性多糖（OS-P），用1-氰基-4-二甲胺基吡啶四氟硼酸盐（CDAP）活化O-SP，己二酸二肼（ADH）作连接臂，在乙基二甲氨基丙基碳二亚胺盐酸盐（ EDAC）作用下，与破伤风类毒素（ TT）结合制备出O-SP-TT结合物，并对该结合物进行添加Al（OH）3佐剂和不添加该佐剂的小鼠免疫原性试验和免疫保护性试验。结果加有Al（OH）3佐剂的结合物在小鼠免疫原性试验中，产生了高效价的IgG抗体，与不添加该佐剂试验组相比，差异具有统计学意义（P＜0．05）；免疫保护性试验表明，添加Al（OH）3佐剂结合物免疫小鼠对5～10 LD50活菌的腹腔攻击具有良好的保护作用。结论 Al（ OH）3佐剂对本结合物有优良的免疫增强作用，添加该佐剂的结合物可望有效防止IATS 11型铜绿假单胞菌感染。%Objective To investigate effect of Al ( OH) 3 adjuvant on immunogenicity and immune protection of a conjugate composed of Pseudomonas aeruginosa(IATS 11)O-specific polysaccharide bounds with tetanus toxoid .Method Lipopo-lysaccharide (LPS) from Pseudomonas aeruginosa bacteria (IATS 11) was isolated via hot phenol extraction and then hy-drolyzed to obtain O-specific polysaccharide (O-SP).O-SP was activated with CDAP,using adipic acid dihydrazide as a linker.Activated O-SP was conjugated to tetanus toxoid via EDAC mediated condensation reaction .Immunogenicity of the conjugate with Al ( OH) 3 adjuvant or without the adjuvant and its immune protection were evaluated in mice .The challenge test was conducted in mice immunized with the conjugate .Results Significantly high IgG anti-IATS 11 O-SP levels were induced in the mice immunized with the conjugates with Al ( OH) 3 adjuvant comparing with that of immunized with the con-jugates without the adjuvant (P<0.05).The potency study showed that more than 80% of the mice immunized with the conjugate with the adjuvant survived when challenged with bacteria of 5-10 LD50 .Conclusion Al ( OH ) 3 adjuvant showed that great immunopotentiation and the conjugate with the adjuvant is expected to effectively against IATS 11 Pseudo-monas aeruginosa.
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