钝顶螺旋藻营养生理的研究rnII．钝顶螺旋藻对无机氮的吸收利用

摘要

利用均匀设计法设计得到的12种培养基及对照Zarrouk培养基对钝顶螺旋藻(Spirulina platensis)S6品系进行培养，研究了在不同培养基下螺旋藻对无机氮的吸收利用。结果表明，螺旋藻可以同时以NO3-N 和NH4-N为氮源。NO3-N对螺旋藻是最为通用和安全的氮源，但添加浓度以11mmol/L左右最为适宜，既可满足藻体的最佳生长需求又可降低养殖成本；适宜浓度的NH4-N可促进螺旋藻的生长，浓度过高则会造成NH3中毒，NH4-N的添加量以1.27～2.57mmol/L范围最为适宜。%Cultured with twelve media designed with the uniform design method, in comparison with the zarrouk medium,the uptake of inorganic nitrogen(NO3-N and NH4-N) by the blue-green algae Spirulina platensis strain S6 are studied in this paper. Result shows that the Spirulina can use both NO3-N and NH4-N as nitrogen sources at the same time. NO3-N is the most popular and safest nitrogen source to Spirulina, the suitable concentration is about 11mmol/L, which can speed the growth rate and reduce the cost of cultivation as well. NH4-N of the suitable concentration can enhance the growth of Spirulina，but overhigh concentration will cause the ammoniac toxicosis, the suitable concentration of NH4-N is about 1.27～2.57mmol/L.