灯盏花素注射液对大鼠肺纤维化干预效果研究

摘要

Objective:To investigate the effect of Breviscapine Injection on changes of in oxyradical contents and microstructure of the pulmonary tissue of sprague-dawley(SD)rats with pulmonary fibrosis induced by pouring bleomycin into the lungs.Methods:Forty pulmonary fibrosis rats that were poured bleomycin into lungs were randomLy divided into four groups:contro,l bleomycin(BLM),breviscapine(BRE) and dexamethasone(DXM) group,with 10 rats in each group.After 7,28 days,the HYP、MDA and SOD of lung were measured,and the histology was examined.Results:In the BRE group,the MAD was significantly lower and the SOD significantly highter than which of BLM group,and the degree of fibrosis was more mitigative than BLM group when examined by microscope,similar to that in the DXM group.Conclusion:breviscapus may be useful in inhibiting pulmonary fibrosis,and can reduce the damage caused by oxygen-derived free radicals in bleomycin.%目的:探讨灯盏花素注射液对博来霉素引起肺纤维化SD大鼠肺组织氧自由基和显微结构的影响.方法:40只SD大鼠经气管内灌注博莱霉素诱导肺纤维化,随后腹腔注射灯盏花素注射液10 mg/kg,另一组注射醋酸地塞米松注射液.注药后于7,28 d各处死一半.通过HE染色和透射电镜检查肺组织,并测定肺组织HYP、SOD和MDA来评价干预效果.结果:灯盏花素注射液组与地塞米松注射液组结果相似,与博莱霉素组相比,肺组织SOD升高,MDA下降,光镜和电镜结果提示肺纤维化程度减轻.结论:灯盏花素注射液能减轻大鼠肺纤维化,部分机制与抗氧化有关.