胃癌组织中幽门螺旋杆菌感染与 Cox -2及 TiaMl 表达的关系研究

摘要

目的：通过分析不同病理类型组织胃癌中幽门螺旋杆菌的感染情况、病灶组织中环氧合酶2（Cox -2）及 T淋巴瘤侵袭转移因子（TiaMl）的表达情况，探讨在各类型胃癌的病理组织中幽门螺旋杆菌感染与 Cox -2，TiaMl 表达的相关性。方法：选择90例不同病理类型胃癌患者作为研究组，并将22例健康志愿者作为对照组。用 HE 染色法及乳胶凝集试验检测血中抗 Hp IgG 抗体的方法评估 Hp 的感染情况，原位聚合酶链反应（PCR）技术及免疫组织化学技术检测病灶组织中 Cox -2，TiaMl 的蛋白表达情况。用多元线性回归分析，得出 Hp 及 Cox -2，TiMal 的相关性。结果：病灶组织中 Cox -2 及 TiMal 的蛋白表达量值随着 Hp 抗体浓度的升高而增加，二者具有正相关性。结论：Hp 与 Cox -2，TiMal 蛋白表达量成正相关性，通过检测血清 Hp 抗体，Cox -2，TiMal 的表达，可为临床胃癌的诊断提供参考。%Objective:To explore the relationship between Helicobacter pylori infection in various types of gastric patho-logical tissue and the expression of Cox - 2 and TiaMl by analyzing the different types of gastric pathological tissue infected by H. pylori and the exprssion levels of cyclooxygenase 2 and T lyMphoMa invasion and Metastasis factor. Methods:90 cases of gastric cancer patients with different pathological types were considered as expriMental group,22 cases of norMal patients as control group. HE staining and exprssion level of anti - Hp IgG detected by Hp latex agglutination test were used to analyze Hp infection. Situ polyMerase chain reaction Method(PCR)technology and iMMunohistocheMistry were perforMed to detect the protein expression of Cox - 2 and TiaMl. Multiple linear regression analysis evaluated the relationship between Hp and Cox - 2. Results:With the increase of Hp antibody concentration,the protein expression levels of Cox - 2 and TiMal were trend of in-crease in nidus organization,which had a positive correlation. Conclusion:There is a positive relationship between Hpinfection and the expression levels of Cox - 2 and TiMal protein. Analyzing the levels of Hp seruM antibody,Cox - 2 and TiMal May pro-vide an iMportant reference for the diagnosis of gastric cancer in clinical.