抑癌基因PTEN、PI3K、AKT在肺癌中的表达及临床意义

摘要

Objective The purpose of this study is to discuss the expression and pathological signifi-cance of tumor suppressor genes PTEN ,PI3K and AKT in lung cancer .Methods SP immunohistochemical meth-od was used to detect the expressions of PTEN ,PI3K and AKT in 62 cases of lung cancer tissues and 30 cases of cancer adjacent tissues ,and analyzed the correlation and relationship with clinical pathological features .The aim was to explore their roles in lung cancer mechanism .Results The mRNA expression of PTEN in lung cancer tis-sue was significantly lower than that in cancer adjacent tissue ,whereas,the mRNA expressions of PI3K and AKT were significantly higher than that in cancer adjacent tissue (P<0.05).Positive expression rate of PTEN in lung cancer tissues was 48.39%(30/62),which was significantly lower than that of cancer adjacent tissue .The posi-tive expression rate of PI3K and AKT in lung cancer tissue were 79.03%(49/62)and 70.97%(44/62),which was significantly higher than that in cancer adjacent tissue (P<0.01).The expressions of PTEN and PI3K were related with the degree of differentiation ,TNM stage,lymph node metastasis of lung cancer and pathological types . AKT expression was related with the TNM stage ,lymph node metastasis and pathological types of lung cancer ( P<0.05).PI3K and AKT expression were positively correlated (P<0.05),PI3K and PTEN expression were negatively correlated(P<0.05),AKT and PTEN expression were negatively correlated (P<0.05).Conclusion The deceased expression of PTEN tumor suppressor gene in lung cancer and increased expression of PI 3K/AKT,prompt the PTEN and PI3K,and AKT may promote the occurrence ,development and metastasis of lung cancer,and PI3K/AKT signal pathways play an important role in the process ,the gene targeted therapy of lung cancer may provide a new direction .%目的：探讨抑癌基因PTEN、PI3K、AKT在肺癌中的表达及临床病理学意义研究。方法采用免疫组化SP法检测PTEN、PI3K和AKT蛋白在62例肺癌组织和30例癌旁肺组织中的表达情况，并分析两者的相关性及与临床病理特征的关系，探讨其在肺癌中的作用机制。结果 PTEN mRNA在肺癌组织中表达明显低于癌旁组织，但PI3K mRNA和AKT mRNA在肺癌组织中表达则高于癌旁组织，差异均有统计学意义（P＜0．05）。 PTEN在肺癌组织中的阳性表达率为48．39％（30/62），明显低于癌旁组织，而PI3K和AKT在肺癌组织中的阳性表达率分别为79．03％（49/62）和70．97％（44/62），明显高于癌旁组织，差异具有统计学意义（ P＜0．01）。 PTEN、PI3K表达与肺癌的分化程度、TNM分期、淋巴结转移及病理类型相关，而AKT表达与肺癌的TNM分期、淋巴结转移及病理类型相关（ P＜0．05）。 PI3K与AKT表达呈正相关（P＜0．05），与PTEN表达呈负相关（P＜0．05），AKT与PTEN表达呈负相关（P＜0．05）。结论抑癌基因PTEN在肺癌中的低表达和PI3K/AKT的高表达，提示了PTEN、PI3K及AKT可能促进了肺癌的发生、发展及转移，而PI3 K/AKT细胞信号通路在此过程中起重要作用，可为肺癌的基因靶向治疗提供一个新的方向。