Objective To discuss the effect of vitamin E on lung development and vascular remodeling in fetal rats with congenital diaphragmatic hernia (CDH). Methods Eighteen pregnant SD rats were randomly divided into 3 groups; control group, CDH group and intervention group. Rats in CDH group and intervention group underwent intragastric administration with nitrofen 125 mg ( soluble in olive oil 2 ml). Rats in control group were given olive oil 2 ml. Rats in intervention group were given vitamin E 140 mg/kg ( soluble in alcohol 2 ml) q. D for 3 days from the 11 th day of pregnancy. CDH group and intervention group were given alcohol 2 ml. Results Incidence of teratogenesis caused by diaphragmatic hernia in intervention group was less than that in CDH group (46. 34% vs 66. 67% , P<0. 05 ). Fetal rats in control group and CDH group showed similar lung development. The lung weight/body weight ratio, axial gland alveolus count ( RAC) , pulmonary alveolus area proportion and pulmonary alveolus area ratio in intervention group were significantly higher than those in CDH group [(3.45±0.58) mg/g vs (2.36±0.61) mg/g,4.4±0.3 vs2.3±0.2, (63.7±5.6)% vs ( 46. 5 ±7. 3) % , (45 ±6)% vs(27±6.7)%,P<0.01]. The alveolar septum area ratio in intervention group was significantly lower than that in CDH group [(45±6.8)% vs (63±8.3)% , P < 0.01]. The mean media thickness percentage of pre-acinar pulmonary arteries and that of intra-acinar pulmonary arteries of fetal rats in intervention group were significantly lower than those in CDH group [(22. 1±2.2)% vs (24.3±4.0)% , (17.6±2.8)% vs (21.5±2.9)% , P < 0.01 ]. The proportion of non-muscle arteries in IAPA per unit of visual field in intervention group was significantly higher than that in CDH group [(79.4±3.2)% vs (71.6±3.3)%, P< 0.01]. Conclusions Prenatal treatment with vitamin E can decrease the incidence of CDH, which may improve the lung development and inhibit the vascular remodeling in lung.%目的 探讨维生素E对先天性膈疝(CDH)大鼠模型胎仔肺发育不良和肺血管重构的影响.方法 将18只SD雌鼠定时配种成功后分为对照组、膈疝组和干预组,以除草醚诱导建立大鼠CDH动物模型,并以维生素E进行干预,第21 d剖宫取出胎鼠及其肺组织,进行光镜及电镜观察,结果进行图像及统计学分析.结果 干预组膈疝致畸率46.34％小于膈疝组致畸率66.67％ (P＜ 0.05).干预组胎肺发育接近对照组,其肺重/体重值[(3.45±0.58) mg/g]、轴向腺泡计数(4.4±0.3)、肺泡面积比例[(63.7±5.6)％]和肺泡面积比[(45±6)％]均大于膈疝组[ (2.36±0.61) mg/g、(2.3±0.2)％、[(46.5±7.3)％]、[(27±6.7)％],P＜ 0.01],而肺泡间隔面积比[(45±6.8)％]低于膈疝组[(63±8.3)％,P＜0.01]；同时腺泡前内动脉(PAPA/IAPA)的平均中膜厚度百分比[(22.1±2.2)％、(17.6±2.8)％]低于膈疝组[(24.3±4.0)％、(21.5±2.9)％,P＜0.01]；单位视野内腺泡内动脉(IAPA)非肌型血管的比例[(79.4±3.2)％]高于膈疝组[(71.6±3.3)％,P＜0.01],小动脉的肌化受到抑制.结论 维生素E产前干预能降低CDH大鼠模型胎仔膈疝的发生率,并且有助于促进CDH胎仔肺的发育,抑制其肺血管重构.
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