吸烟对肺癌患者血清转化生长因子β和白介素10水平的影响研究

摘要

目的：探讨吸烟对肺癌患者血清转化生长因子β（ TGF-β）和白介素10（ IL-10）水平的影响及癌组织TGF-β、IL-10表达与患者临床病理特征的关系。方法选择2013年10月—2014年10月在华南农业大学医院治疗的肺癌患者94例，根据患者吸烟情况分成吸烟组和非吸烟组，各47例。采用双抗体夹心ABC-ELISA法测定血清TGF-β、IL-10水平，比较两组患者血清TGF-β、IL-10水平及肺功能〔用力肺活量（ FVC）、第一秒用力呼气容积（FEV1）、1秒率（FEV1/FVC%）、最大呼气中期流量（MMEF）、用力呼气75%肺活量（FEF75%）、用力呼气50%肺活量（ FEF50%）〕，并分析癌组织TGF-β、IL-10表达与肺癌患者临床病理特征的关系。结果吸烟组患者血清 TGF-β水平高于非吸烟组（P<0.01）；但两组患者血清IL-10水平比较，差异无统计学意义（P>0.05）。吸烟组和非吸烟组不同性别、年龄、病理类型、分化程度及有无远处转移患者癌组织TGF-β、IL-10阳性率比较，差异无统计学意义（P>0.05）；不同TNM分期及有无淋巴结转移患者癌组织TGF-β、IL-10阳性率比较，差异有统计学意义（ P<0.05）。两组患者 FEV1/FVC%比较，差异无统计学意义（ P >0.05）；但吸烟组患者 FVC、FEV1、MMEF、FEF75%及FEF50%均低于非吸烟组（ P<0.05）。结论吸烟的肺癌患者血清TGF-β水平明显升高，肺功能明显降低，但未发现吸烟对血清IL-10水平的影响；检测血清TGF-β水平有助于判断肺癌TNM分期及有无淋巴结转移。%Objective To investigate the impact of smoking on serum levels of TGF-β and IL-10 in patients with lung cancer,and to analyze the relationship between expression of TGF-β and IL-10 of cancer tissues and clinicopathologic features. Methods A total of 94 patients with lung cancer were selected in South China Agriculture University Hospital from October 2013 to October 2014 , and they were divided into smoking group and non - smoking group according to smoking behaviors,each of 47 cases. ABC-ELISA was used to detect the serum levels of TGF-β and IL-10,and the lung function index including FVC,FEV1 ,FEV1/FVC%,MMEF,FEF75%,FEF50% were compared between the two groups. Results Serum TGF-β level of smoking group was statistically significantly higher than that of non-smoking group ( P<0. 05 );while no statistically significant differences of serum IL-10 level was found between the two groups(P>0. 05). In both groups,no statistically significant differences of positive expression rate of TGF-β or IL-10 was found in patients with different gender, ages,pathological types,differentiation degrees,with or without distant metastasis(P>0. 05);while there were statistically significant differences of positive expression rate of TGF-β and IL-10 in patients with different TNM stages,with or without lymphatic metastasis(P<0. 05). No statistically significant differences of FEV1/FVC% was found between the two groups(P>0. 05),while FVC,FEV,MMEF,FEF75% and FEF50% of smoking group were statistically significantly lower than those of non-smoking group ( P <0. 05 ). Conclusion Serum TGF-β level of smoking patients with lung cancer is obviously higher,and the lung function is obviously worse,detection of serum TGF-β level is helpful to judge the TNM stages and lymphatic metastasis;no obvious impact of smoking on serum IL-10 level was found in this study.