KRAS、BRAF基因突变在大肠锯齿状病变中的临床应用

摘要

目的 研究KRAS、BRAF基因突变在大肠锯齿状病变中的临床应用.方法 选取100例大肠锯齿状病例经切除术后的石蜡标本进行研究.应用直接测序技术对KRAS、BRAF基因的突变进行检测分析.结果 大肠锯齿状病变中KRAS基因突变率为40.00%,BRAF基因突变率为6.67%.所有大肠锯齿状病变患者中有40例检出KRAS基因突变,突变率为40.00%,其中KRAS基因12、13密码子突变率分别为30.00%以及10.00%.KRAS基因突变率与患者性别、年龄、病变部位、分化程度以及淋巴结转移等临床病理特征均无关(P>0.05).BRAF基因突变在>65岁患者以及大肠近端情况较多见(P<0.05).结论 随着KRAS、BRAF基因突变的研究不断深入,对指导临床进行随访及药物治疗预后的判定具有重要的临床意义.%Objective To study the clinical application of KRAS and BRAF gene mutation in colorectal serrated lesions . Methods 100 cases of colorectal serrated cases of paraffin embedded specimens after resection were selected .Mutation of KRAS and BRAF gene were detected by direct sequencing analysis .Results The colorectal serrated lesions in the KRAS gene mutation rate were 40%,BRAF gene mutation rate was 6.67%.All patients with colorectal serrated lesions were found in 40 cases of KRAS gene mutation,mutation probability was 40%,of which 12,13 of the KRAS gene mutation rate was 30%and 10%respec-tively.The mutation rate of KRAS gene and the patient 's gender and age ,clinical and pathological features of the lesion ,degree of differentiation and lymph node metastasis were not significantly related (P>0.05).BRAF gene mutation were mainly in patients>65 or with proximal large intestine.(P<0.05).Conclusion With the further study of KRAS,BRAF gene mutation,it has important clinical significance in guiding follow-up and evaluating prognosis .