IL-27基因多态性和血清 IL-27 p28水平与骨肉瘤风险相关性分析

摘要

Objective To evaluate the correlation of L-27 gene polymorphism and serum levels of IL-27p28 and osteo-sarcoma risk.Methods A comparative study of 160 cases of osteosarcoma patients( study group) and 250 healthy patients( con-trol group) were conducted.IL-27 gene-964 A/G,2905 T/G,and 4730 T/C polymorphism received polymerase chain reaction-re-striction fragment length polymorphism determination.ELISA serum levels of IL-27p28.Results Compared with the control group,the serum levels of IL-27p28 of patients with osteosarcoma were significantly lower(P<0.01).Serum levels of IL-27p28 of patients with osteosarcomaⅢ~Ⅳwere lower than belowⅠ~Ⅱpatients(P<0.05).Serum levels of IL-27p28 of patients with metastasis were lowere than non-metastasis patients(P<0.05).IL-27-964 A/G,2905 T/G,4730 T/C genotype and allele fre-quencies and osteosarcoma risk had no correlation(P>0.05).Stratified analysis failed to show the correlation of-964 A/G,2905 T/G,and 4730 T/C polymorphism with clinical stage and metastasis of osteosarcoma(P>0.05).Conclusion Low serum levels of IL-27p28 maybe related with progression of osteosarcoma,but IL-27 gene-964 A/G,2905 T/G,and 4730 T/C polymorphism and their haplotypes are not associated risk of osteosarcoma.%目的：评估IL-27基因多态性和血清IL-27p28水平与骨肉瘤风险的相关性。方法对比研究160例骨肉瘤患者（研究组）和250例健康患者（对照组）。 IL-27基因-964 A／G，2905 T／G，和4730 T／C多态性采用聚合酶链反应-限制性片段长度多态性测定。酶联免疫吸附试验检测血清中IL-27p28的水平。结果与对照组相比，骨肉瘤患者血清IL-27p28水平显著降低（P＜0．01）。Ⅲ～Ⅳ期骨肉瘤患者血清IL-27p28水平低于Ⅰ～Ⅱ期患者（P＜0．05）。同样，转移患者的血清IL-27p28水平低于非转移患者（ P＜0．05）。 IL-27-964 A／G，2905 T／G，4730 T／C的基因型和等位基因频率与骨肉瘤风险无相关性（ P＞0．05）。分层分析也未能显示-964 A／G，2905 T／G，和4730 T／C多态性与临床分期和骨肉瘤的转移存在相关性（P＞0．05）。结论血清IL-27p28低水平可能与骨肉瘤进展有关，但IL-27基因-964 A／G，2905 T／G，和4730 T／C多态性和它们的单倍型不与骨肉瘤的风险相关联。