多胚亚麻的胚珠整体透明与解剖观察

摘要

利用改进的整体透明技术,以观察多胚性的亚麻受精后的胚珠,同时对整体透明方法进行了讨论.方法:利用60℃ 1M氢氧化钠溶液处理植物材料,然后利用10％次氯酸钠溶液透明,于显微镜下进行解剖观察.结果:在显微镜下,能观察到亚麻受精后胚珠内的幼胚,通过解剖还能清楚地观察到胚珠内的双胚的排列方式；并确定供试材料的双胚的发生率为8％.讨论:与传统的整体透明和切片方法相比,本改进的整体透明方法省时省力,并且能够完成整体透明和解剖的3D数据采集,适用于多胚亚麻幼胚的观察.缺点是用DAPI染色DNA效果不好.%In order to observe the fertilized ovules of polyembryonic flax, an improved whole mount-clearing method was developed and discussed. Young seeds were isolated and immerged in 1 M NaOH solution under the condition of 60cC, then cleared in 10% NaCIO solution. And the young embryos inside the fertilized ovules were clearly visible by a binocular microscopy. Twin embryos were found in some ovules, the frequency of occurrence of twin embryos was 8% of these investigated polyembryonic flaxes. Compared with the traditional whole mount-clearing and chip technologies, this improved method could finish clearing in a short time and provide clear observation results so as to get the corresponding 3D data, and it was suitable for observing the young embryos of polyembryonic flax. However, there was no good result for DAPI staining.